Product Name:
ErbB2-pY735
Product Number:
ab-pk614
Target Full Name: ErbB2 (Neu) receptor-tyrosine kinase
Target Alias: CD340; C-erbB-2; Epidermal growth factor receptor-related protein; ErbB2; HER2; HER-2; Kinase ErbB2; MLN 19; V-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); TKR1; hCG_28177; ENSG00000141736; Q9NP09; Q9UK79
Product Type Specific: ErbB2 protein kinase phosphosite-specific antibody
Antibody Code: PK614
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y735
Protein UniProt: P04626
Protein SigNET: ErbB2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ErbB2 (HER2, Neu) sequence peptide Cat. No.: PE-04AMC95
Antibody Immunogen Sequence: GTV(pY)KGIW(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G732 to W739; in the region between the Transmembrane and kinase catalytic domains. Y735 phosphorylation induces interaction with SHC1. This is one of the major in vivo phosphorylation sites in ErbB2 (≥160 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 138, 175-200 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human ErbB4. No significant cross-reactive proteins detected in A431 cells, except for weak ~75, 50 and 37 kDa cross-reactive proteins.
Related Product 1: ErbB2 (732-739) pY735 antibody blocking peptide (Cat. No.: PE-04AMC95)
Related Product 2: ErbBSubtide kinase substrate peptide (Cat. No.: PE-01BHG95)
Related Product 3: ErbB2 expression antibody (Cat. No.: AB-NK054-4P)
Related Product 4: ErbB2 expression antibody (Cat. No.: AB-NK054-4)
Related Product 5: ErbB2 expression antibody (Cat. No.: AB-NK054-5)
Related Product 6: ErbB2 expression antibody (Cat. No.: AB-NK054-6)
Related Product 7: ErbB2 expression antibody (Cat. No.: AB-NK054-7)
Related Product 8: ErbB2 Y877 phosphorylation antibody (Cat. No.: AB-PK125)
Related Product 9: ErbB2 Y877 phosphorylation antibody (Cat. No.: AB-PK615)
Related Product 10: ErbB2 Y1248 phosphorylation antibody (Cat. No.: AB-PK613)
Scientific Background: ErbB2 (HER2; Neu; CD340) is a 138-185 kDa protein-tyrosine kinase of the TK group and EGFR family. It features an extracellular ligand-binding domain (which binds no ligand), a transmembrane domain, and an intracellular tyrosine kinase domain. It is a kinase that function as a component of several membrane protein receptor complexes on the cell surface. Specifically, the ErbB2 protein functions in the regulation of the growth and stabilization of peripheral microtubules, as well as in the transcriptional activation of several rRNA genes (mediated by RNA polymerase I) that result in enhanced protein synthesis and subsequent cell growth. It is commonly expressed in most tested human tissues except in the brain and spinal cord, where it is more weakly expressed. Protein interaction is induced with phosphorylation of Tyr-1139 (with Grb2, Grb7 & Src), Tyr-1196 (with Crk), and Tyr-1222 (with CGI-27, Grb2 & Shc1). Phosphorylation of Tyr-1112 induces interaction with Cbl, which promotes ErbB2 degradation. Overexpression of unaltered ErbB2 coding sequences in NIH-3T3 cells results in cellular transformation and tumorigenesis. ErbB2 is amplified in 15-30% of primary human breast malignancies and overexpression of ErbB2 is associated with the most aggressive tumours that show uncontrolled proliferation, resistance to apoptosis and increased motility. ErbB2 has also been linked with the development of ovarian cancer, gastric and lung adenocarcinomas, and glioblastomas. Insertional mutagenesis studies in mice also support a role for this protein kinase in mouse cancer oncogenesis. This description may include information annotated by UniProt and/or Google AI.