Product Name:
EphB4-pY596
Product Number:
ab-pk612
Target Full Name: Ephrin type-B receptor 4 protein-tyrosine kinase
Target Alias: EPB4; EPH receptor B4; HTK; Mdk2; MYK1; Tyro11; Tyrosine-protein kinase receptor HTK
Product Type Specific: EphB4 protein kinase phosphosite-specific antibody
Antibody Code: PK612
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y596
Protein UniProt: P54760
Protein SigNET: EphB4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EphB4 sequence peptide Cat. No.: PE-04AMB95
Antibody Immunogen Sequence: PFT(pY)EDP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues P593 to P599; in the EphA2_TM region between the transmembrane and kinase catalytic domains. Y596 phosphorylation stimulates phosphotransferase activity. This is one of the major in vivo phosphorylation sites for EphB4 (≥223 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.08 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and MCF7 cells, except for a very strong ~40 KDa cross-reactive protein in both cells.
Related Product 1: EphB4 (593-599) pY596 antibody blocking peptide (Cat. No.: PE-04AMB95)
Related Product 2: EphB1 Y594 phosphorylation antibody (Cat. No.: AB-PK609)
Related Product 3: EphB2 expression antibody (Cat. No.: AB-NK267-1)
Related Product 4: EphB2 expression antibody (Cat. No.: AB-NK267-2)
Related Product 5: EphB2 Y780 phosphorylation antibody (Cat. No.: AB-PK610)
Related Product 6: EphB3 Y600 phosphorylation antibody (Cat. No.: AB-PK611)
Related Product 7: EphA1 Y599 phosphorylation antibody (Cat. No.: AB-PK928)
Related Product 8: EphA1 Y781 phosphorylation antibody (Cat. No.: AB-PK605)
Related Product 9: EphA2 Y588 phosphorylation antibody (Cat. No.: AB-PK606)
Related Product 10: EphA2 Y772 phosphorylation antibody (Cat. No.: AB-PK607)
Scientific Background: EphB4 is a receptor protein-tyrosine kinase of the TK group and Eph family. It features an N-terminal extracellular ligand-binding domain (composed of a globular domain, a cysteine-rich region, and two fibronectin type III repeats), a single transmembrane segment, and a cytoplasmic tyrosine kinase domain containing a SAM domain and a PDZ-binding motif. It binds the ephrin-B (EPNB) family of ligands (ephrin-A1, B2, or B3) on adjacent cells to mediate cell-contact signalling. It plays a critical role in vascular development, and angiogenesis. It acts as a cell surface receptor for mediating bidirectional signaling, controlling cell migration, adhesion, and separation. It is crucial for heart morphogenesis and arteriovenous differentiation during blood vessel development. EphB4 forward signaling typically promotes cellular repulsion and organization, separating EphB4-expressing cells from ephrin-B2-expressing cells. EFNB2 binding to EphB4 can be inhibited with a L95R mutation. High expression of EphB4 is often associated with tumorigenesis, particularly in colorectal, gastric, and prostate cancers, where it promotes proliferation and angiogenesis. Mutations in the gene can lead to autosomal dominant lymphatic-related hydrops fetalis, a dangerous condition affecting lymphatic system development. This description may include information annotated by UniProt and/or Google AI.