Product Name:
EphB2-pY780
Product Number:
ab-pk611
Target Full Name: Ephrin type-B receptor 2 protein-tyrosine kinase
Target Alias: CEK5; DRT; Eph receptor B2; Eph3; Eph-3; EphB2; Ephrin type-B receptor 2; EphT3; Hek5; Tyro5; MGC87492; ENSG00000133216
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK610
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y780
Protein UniProt: P29323
Protein SigNET: P29323
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EphB2 sequence peptide Cat. No.: PE-04ALZ95
Antibody Immunogen Sequence: DPT(pY)TSA(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D777 to A783; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. This is the major in vivo phosphorylation site in EphA2.
Production Method: Corresponds to amino acid residues D777 to A783; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. This is the major in vivo phosphorylation site in EphA2.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-115 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in MCF7 and HepG2 cells, except for a medium ~75 kDa cross-reactive protein in both cell lines.
Related Product 1: EphB2-pY780 blocking peptide
Related Product 2: EphB1-pY594 phosphosite-specific antibody (Cat. No.: AB-PK609)
Related Product 3: EphB2-BKCD pan-specific antibody (Cat. No.: AB-NK267-1)
Related Product 4: EphB2-PCT pan-specific antibody (Cat. No.: AB-NK267-2)
Related Product 5: EphB3-pY600 phosphosite-specific antibody (Cat. No.: AB-PK611)
Related Product 6: EphSubtide - EphB1 protein kinase substrate peptide
Scientific Background: EphB3 is a member of the TK group of protein kinases in the Eph family. EphB3 is expressed during embryonic development in multiple regions of the central nervous system. In adult brain, EphB3 is expressed in the cerebellum, raphe pallidus, hippocampus, entorhinal cortex, and both motor and sensory cortices, and is moderate to highly expressed in most tested adult human tissues. Orthologues of EphB3 are highly conserved in vertebrates, including amphibians. EphB3 is activated by binding ephrin-B1, B2, or B3. Phosphorylation at Y614 induces interaction with Crk, Fyn, and RasGAP. EphB3 is involved in the maintenance of mature neuronal connections and/or re-arrangement of synaptic connections during late stages of development. EphB3 plays a role in the regulation of cell adhesion and migration, and the catalytic activity of EphB3 is required for inhibition of integrin-mediated cell adhesion. EphB3 appears to be an oncoprotein (OP). Over-expression of EphB3 has been observed in several cancer types, including colon cancer and lung cancer. Additionally, EphB3 over-expression was demonstrated in non-small cell lung cancer (NSCLC) and the expression level was highly correlated with pathological features, including tumour size, differentiation, and metastasis. over-expression of EphB3 in NSCLC cell lines enhanced cell growth and migration and promoted tumourigenesis. By contrast, EphB3 knockout in NSCLC lines reduced cell proliferation and migration and suppressed tumour growth. EphB3 has been linked with the development of lung neuroendocrine carcinomas and lung small cell carcinomas (LSCC).
