Product Name:
EphA1-pY781
Product Number:
ab-pk607
Target Full Name: Ephrin type-A receptor 1 protein-tyrosine kinase
Target Alias: EPA1; Eph; Eph receptor A1; Ephrin type-A receptor 1; EphT; EphT1; MGC163163; Tyrosine-protein kinase receptor Eph; CCDS5884.1; P21709; A1L3V3; ENSG00000146904
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK605
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y781
Protein UniProt: P21709
Protein SigNET: P21709
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EphA1 sequence peptide Cat. No.: PE-04ALV80
Antibody Immunogen Sequence: DGT(pY)ETQ(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D778 to Q784; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues D778 to Q784; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-strong immunoreactivity of a target-sized protein by Western blotting in HeLa cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-105 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: At least 7 other cross-reactive proteins detected in HeLa cells.
Related Product 1: EphA1-pY781 blocking peptide
Related Product 2: EphA2-pY588 phosphosite-specific antibody (Cat. No.: AB-PK606)
Related Product 3: EphA3-pY779 phosphosite-specific antibody (Cat. No.: AB-PK608)
Scientific Background: EphA1 is a protein-tyrosine kinase of the TK group and Eph family. It is activated by binding of ephrin-A1 on a presenting cell, which is likely to induce dimerization and autophosphorylation. This kinase is moderate to highly expressed in most tested human tissues. Orthologues of EphA1 are highly conserved in mammals and birds. EphA1 has been implicated in mediating developmental events, particularly in the nervous system. EphA1 seems to be a marker of the differentiated normal epidermis, and its downregulation in nonmelanoma skin cancer may contribute to carcinogenesis of these very frequent human tumors. EphA1 represents a new potential prognostic marker and therapeutic target in nonmelanoma skin cancer. EphA1 has also been linked with the development of glioblastomas, head and neck cancer and breast carcinomas (pleomorphic lobular).
