Product Name:
EIF2AK3-pT982
Product Number:
ab-pk606
Target Full Name: Eukaryotic translation initiation factor 2-alpha kinase 3
Target Alias: DKFZp781H1925; E2AK3; EIF2AK3; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; HRI; Kinase PEK; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK604
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T982
Protein UniProt: Q9NZJ5
Protein SigNET: Q9NZJ5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EIF2AK3 (PERK) sequence peptide Cat. No.: PE-04AON99
Antibody Immunogen Sequence: ARH(pT)GQV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A889 to V895; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues A889 to V895; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in MCF7 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: ~50 kDa cross-reactive protein in MCF7 cells and an ~80 kDa protein in HeLa cells.
Related Product 1: EIF2AK3-pT982 blocking peptide
Scientific Background: PERK (EIF2AK3) is a protein-serine/threonine kinase of the Other group and PEK family. It serves as a critical effector of unfolded protein response-induced G1 growth arrest. Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and induction of its phosphotransferase activity.
