Product Name:
EIF2AK3-pT982
Product Number:
ab-pk604
Target Full Name: Eukaryotic translation initiation factor 2-alpha kinase 3
Target Alias: DKFZp781H1925; E2AK3; EIF2AK3; Eukaryotic translation initiation factor 2-alpha kinase 3; HsPEK; HRI; Kinase PEK; Pancreatic eIF2-alpha kinase; PRKR-like endoplasmic reticulum kinase
Product Type Specific: EIF2AK3 protein kinase phosphosite-specific antibody
Antibody Code: PK604
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T982
Protein UniProt: Q9NZJ5
Protein SigNET: EIF2AK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EIF2AK3 (PERK) sequence peptide Cat. No.: PE-04AON99
Antibody Immunogen Sequence: ARH(pT)GQV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A889 to V895. T982 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is phosphorylation site in EIF2AK3 has not been reported from high throughput mass spectrometry studies recorded in PhosphoSitePlus. EIF2AK3 is known to be phosphorylated at this site in vitro by PERK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in MCF7 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human (The amino acid residues in this phosphosite are not well conserved).
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: ~50 kDa cross-reactive protein in MCF7 cells and an ~80 kDa protein in HeLa cells.
Related Product 1: PERK (889-895) pT982 antibody blocking peptide (Cat. No.: PE-04AON99)
Related Product 2: eIF2⍺ S52 phosphorylation antibody (Cat. No.: AB-PN820)
Related Product 3: EIF2AK1 S258 phosphorylation antibody (Cat. No.: AB-PK876)
Related Product 4: EIF2AK3 S555 phosphorylation antibody (Cat. No.: AB-PK926)
Related Product 5: EIF2AK3 S715 phosphorylation antibody (Cat. No.: AB-PK927)
Related Product 6: EIF2AK4 T667 phosphorylation antibody (Cat. No.: AB-PK877)
Related Product 7: eIF4B S504 phosphorylation antibody (Cat. No.: AB-PN819)
Related Product 8: eIF4E S209 phosphorylation antibody (Cat. No.: AB-PN956)
Related Product 9: eIF4G1 T205+T207 phosphorylation antibody (Cat. No.: AB-PN1008)
Related Product 10: eIF4G1 S1185+S1187 phosphorylation antibody (Cat. No.: AB-PN1004)
Scientific Background: PERK (Protein kinase R-like endoplasmic reticulum kinase; EIF2AK3) is a protein-serine/threonine kinase of the Other group and PEK family. It serves as a critical effector of unfolded protein response-induced G1 growth arrest. Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and induction of its phosphotransferase activity. It mitigates ER stress by phosphorylating eIF2α to reduce protein synthesis and activating survival pathways, playing a crucial role in pancreatic beta-cell function. Under ER stress, the chaperone GRP78 dissociates from PERK, allowing it to oligomerize and autophosphorylate, which activates its cytosolic kinase domain. It features an N-terminal ER luminal stress-sensing domain, a transmembrane segment, and a C-terminal cytosolic kinase domain. It phosphorylates the alpha-subunit of eukaryotic initiation factor 2 (eIF2a) at Ser-51. This action reduces the load of unfolded proteins entering the ER by inhibiting general translation initiation. While global translation is reduced, PERK activation specifically increases the translation of transcription factors like ATF4, which promote cell survival and antioxidant responses. Mutant TDP-43 transgenic flies showed increased eIF2a phosphorylation and an impaired motor function (1). In line with this, knockdown of the fly homologue of PERK improved the motor function in these flies (2). PERK is implicated in pancreatic development, with mutations causing Wolcott-Rallison syndrome. It is also involved in promoting survival in cancer cells and modulating neurodegenerative diseases. This description may include information annotated by UniProt and/or Google AI.