Product Name:

DNAPK-pT2609


Product Number:

ab-pk597

Price:

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$89.00
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Target Full Name: DNA-dependent protein kinase catalytic subunit

Target Alias: DNA-PK catalytic subunit; DNA-PKcs; DNPK1; P460; PRKD; PRKDC; XRCC7; HYRC; HYRC1; p350; ENSG00000121031

Product Type Specific: Protein kinase phosphosite-specific antibody

Antibody Code: PK595

Antibody Target Type: Phosphosite-specific

Antibody Phosphosite: T2609

Protein UniProt: P78527

Protein SigNET: P78527

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human DNAPK (PRKDC) sequence peptide Cat. No.: PE-04ALT95

Antibody Immunogen Sequence: FVT(pT)QAS(bA)C

Antibody Immunogen Description: Corresponds to amino acid residues F2606 to S2612; In the mid-region of the protein kinase between the NUC194 and FAT domains.

Production Method: Corresponds to amino acid residues F2606 to S2612; In the mid-region of the protein kinase between the NUC194 and FAT domains.

Antibody Modification: Protein kinase phosphosite-specific antibody

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 460-480 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa cells.

Scientific Background: DNAPK (PRKDC) is a protein-serine/threonine kinase of the Atypical group and PIKK family. Phosphorylation at -612, T2609, T2638, T2647 and T3950 inhibits phosphotransferase activity. DNAPK has been noted to be involved in sensing DNA damage, including DNA non-homologous end joining (NHEJ) and therefore, by association, be involved with V(D)J recombination events and double-strand break (DSB) repair. A possible scaffolding role of DNAPK for DNA-repair enzymes has also been proposed. DNAPK appears to be a tumour suppressor protein (TSP). The active form of the protein kinase normally acts to inhibit tumour cell proliferation until damage to DNA can be repaired. Therefore, it can play a role to facilitate tumour cell survival under conditions of radiation and chemotherapy.