Product Name:
DDR2-pY736
Product Number:
ab-pk593
Target Full Name: Discoidin domain-containing receptor 2
Target Alias: Discoidin domain receptor 2; Discoidin domain receptor tyrosine kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3; Receptor protein-tyrosine kinase TKT; TKT; TYRO10; Tyrosine-protein kinase TYRO 10; MIG20a; ENSG00000162733
Product Type Specific: DDR2 protein kinase phosphosite-specific antibody
Antibody Code: PK593
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y736
Protein UniProt: Q16832
Protein SigNET: DDR2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DDR2 (Tyro10) sequence peptide Cat. No.: PE-04AHB95
Antibody Immunogen Sequence: RNL(pY)SGD(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R733 to D739. Y736 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major in vivo phosphorylation site in DDR2 (≥414 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). DDR2 is known to be phosphorylated at this site in vitro by Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-95 kDa.
Related Product 1: DDR2 (733-739) pY736 antibody blocking peptide (Cat. No.: PE-04AHB95)
Related Product 2: DDR2Subtide kinase substrate peptide (Cat. No.: PE-01BHB90)
Related Product 3: DDR1 expression antibody (Cat. No.: AB-NK263-1)
Related Product 4: DDR1 Y796+Y797 phosphorylation antibody (Cat. No.: AB-PK591)
Related Product 5: DDR1 Y797 phosphorylation antibody (Cat. No.: AB-PK592)
Related Product 6: DDR2 Y740 phosphorylation antibody (Cat. No.: AB-PK594)
Scientific Background: DDR2 (Discoidin Domain Receptor 2; CD167b) is a receptor protein-tyrosine kinase of the TK group and DDR family. It is a receptor kinase that binds fibrillar collagens (types I, III, V) and has roles in the regulation of cell differentiation, extracellular matrix remodeling, cell migration, and cell proliferation. Its extracellular discoidin domain binds specifically to triple-helical collagen motifs, initiating signalling. DDR2 is necessary for normal bone development and promotes fibroblast migration and proliferation, and has a critical role in wound healing. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain where it is poorly expressed. It promotes matrix degradation by inducing collagenases such as MMP-1, MMP-2, and MMP-13. Orthologues of DDR2 are highly conserved in vertebrates and insects. Phosphorylation of DDR2 at Tyr-471 induces interaction with Shc1, whereas phosphorylation at Y740 induces interaction with Nck2 and SHP2. DDR2 isfeatures a distinct extracellular region encompassing a factor VIII-like domain, which is thought to be involved in the regulation of cell growth, differentiation, and metabolism. DDR2 plays a role in the regulation of collagen turnover mediated by smooth muscle cells in obstructive diseases of blood vessels and the lung. It is associated with increased matrix metalloproteinase-13 (MMP-13) activity, contributing to cartilage degeneration. DDR2 has also been linked with spondyloepimetaphyseal dysplasia short limb-hand type (semd-sl). Overexpression or gain-of-function mutations for DDR2 are found in squamous lung large cell carcinomas, breast cancers, and hepatocellular carcinomas, driving cell invasion and metastasis, often via EMT regulation. This description may include information annotated by UniProt and/or Google AI.