Product Name:
DAPK-pS269
Product Number:
ab-pk592
Target Full Name: Mitogen-activated protein kinase kinase kinase 12
Target Alias: Dual leucine zipper bearing kinase; Dual leucine zipper kinase DLK; EC 2.7.11.25; Kinase DLK; Leucine-zipper protein kinase; M3K12; MAP3K12; MAPK-upstream kinase; MEKK12; MUK; ZPK; ZPKP1
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK590
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S269
Protein UniProt: Q12852
Protein SigNET: Q12852
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DLK (ZPK) sequence peptide Cat. No.: PE-04ALS85
Antibody Immunogen Sequence: TKM(pS)FAG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T266 to G272; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues T266 to G272; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity of a target-sized protein by Western blotting in A431 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2 cells and sea star oocytes, except for a ~76 kDa cross-reactive protein and phospho-CDK1 in germinal vesicle-positive, maturing sea star oocytes.
Related Product 1: DAPK-pS269 blocking peptide
Related Product 2: MYL9 (12-23) KinSub - Myosin light chain 9 (A12-S23, human) peptide; PHKG protein kinase substrate peptide
Related Product 3: DAPKSubtide - DAPK3 (ZIPK) protein kinase substrate peptide
Related Product 4: ZIPtide KinSub - DAPK3 (ZIPK) protein kinase substrate peptide
Scientific Background: DLK (MAP3K12) is a protein-serine/threonine kinase of the TKL group and MLK family. It is a protein kinase that has been noted as a possible JNK/SAPK pathway activator. In vivo DLK has been noted to phosphorylate beta-casein, myelin basic protein, and histone 1.
