Product Name:
Csk-pY184
Product Number:
ab-pk591
Target Full Name: C-terminus of Src tyrosine kinase
Target Alias: CYL; MGC117393; MPK-2; CCDS10269.1; ENSG00000103653
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK589
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y184
Protein UniProt: P41240
Protein SigNET: P41240
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Csk sequence peptide Cat. No.: PE-04AGJ95
Antibody Immunogen Sequence: DEF(pY)RSG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D181 to G187; In the region between the SH2 domain and the protein kinase catalytic domain
Production Method: Corresponds to amino acid residues D181 to G187; In the region between the SH2 domain and the protein kinase catalytic domain
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity of a target-sized protein by Western blotting in A431 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-52 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: Strong ~76 kDa cross-reactive protein detected in A431, HeLa and Jurkat cells, as well as a strong ~180 kDa cross-reactive protein in Jurkat cell.
Related Product 1: Csk-pY184 blocking peptide
Related Product 2: CskSubtide - CSK protein kinase substrate peptide
Scientific Background: CSK is a protein-tyrosine kinase of the TK group and Csk family. It is a protein kinase that can regulate cell differentiation, growth, and migration, along with mediating immune responses. A Y184F mutation leads to inhibited phosphorylation of CSK. Activity can be decreased by 2/3 with a Y304F mutation. PRKACA induced phosphorylation of CSK can be inhibited with a CSK mutation S364A.
