Product Name:
DDR1-pY796+pY797
Product Number:
ab-pk591
Target Full Name: Epithelial discoidin domain-containing receptor 1 protein-tyrosine kinase
Target Alias: CAK; CD167; CD167a; CD167a antigen; Cell adhesion kinase; Discoidin domain receptor tyrosine kinase 1; Discoidin receptor tyrosine kinase ;DDR; EDDR1; MCK10; NEP; NTRK4; PTK3; Tyrosine- protein kinase CAK; ENSG00000215522
Product Type Specific: DDR1 protein kinase phosphosite-specific antibody
Antibody Code: PK591
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y796+Y797
Protein UniProt: Q08345
Protein SigNET: DDR1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DDR1 sequence peptide Cat. No.: PE-04ACP99
Antibody Immunogen Sequence: AGD(pY)(pY)RV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A793 to V799; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. Y796 and Y796 phosphorylation stimulate phosphotransferase activity and induce interaction with the protein-tyrosine phosphatase SHP2. Y796 and Y797 are the two major in vivo phosphorylation sites in DDR1 (≥951 and ≥311 reports, respectively, from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.3 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-120 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 and Jurkat cells and sea star oocytes, except for a ~75 kDa protein in FBS-treated A431 cells..
Related Product 1: DDR1 (793-799) pY796+pY797 antibody blocking peptide (Cat. No.: PE-04ACP99)
Related Product 2: DDR2Subtide kinase substrate peptide (Cat. No.: PE-01BHB90)
Related Product 3: DDR1 expression antibody (Cat. No.: AB-NK263-1)
Related Product 4: DDR1 Y797 phosphorylation antibody (Cat. No.: AB-PK592)
Related Product 5: DDR2 Y736 phosphorylation antibody (Cat. No.: AB-PK593)
Related Product 6: DDR2 Y740 phosphorylation antibody (Cat. No.: AB-PK594)
Scientific Background: DDR1 (Discoidin Domain Receptor 1) is a receptor protein-tyrosine kinase of the TK group and DDR family an an adhesion protein. It is a cell surface receptor for fibrillar collagen (activated by collagen types I, II, III, IV, V, and VIII), and regulates cell attachment, cell migration, differentiation, and cell proliferation. It undergoes autophosphorylation in response to fibrillar collagen binding. It exists as ligand-independent dimers, but collagen binding induces downstream signaling, including MAPK pathways and Src activation. Phosphorylation at Tyr-396, Tyr-703 and Tyr-796 induces interaction with SHP2 and Tyr-513 to Shc1. It is mainly expressed by epithelial cells in the kidneys, lungs, gastrointestinal tract, and brain, DDR1 can interact with Notch1 and produce a prosurvival effect. It can also enhance cancer cell migration. Multiple isoforms (DDR1a, 1b, 1c, 1d, 1e) exist due to alternative splicing, with DDR1a and 1b being the most functional. DDR1 may be an oncoprotein (OP) or a tumour suppressor protein (TSP). DDR1 has been shown to be significantly overexpressed in some human tumours. It has been shown to promote the proliferation of neoplastic cells. Multiple sites of mutation lead to either inhibition or constitutively activation of this receptor. The gene expression was found to be upregulated by p53 in human osteosarcoma cells. This description may include information annotated by UniProt and/or Google AI.