Product Name:
CSF1R-pY809
Product Number:
ab-pk590
Target Full Name: Macrophage colony-stimulating factor 1 receptor
Target Alias: CD115; C-fms; Colony stimulating factor 1 receptor; CSF-1-R; CSFMR; FMS; Fms proto-oncogene; Kinase CSFR; Macrophage colony stimulating factor I receptor precursor; M-CSFR; FIM2; FIM2; CCDS4302.1; A2VDG3; Q6LEI2; ENSG00000182578
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK588
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y809
Protein UniProt: P07333
Protein SigNET: P07333
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CSF1R (Fms) sequence peptide Cat. No.: PE-04AKC99
Antibody Immunogen Sequence: DSN(pY)IVK(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D806 to K812; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues D806 to K812; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak reactivity with recombinant human CSF1R on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with recombinant human Flt3 and Kit, which also feature nearly an identical phosphosite sequence.
Related Product 1: CSF1R-pY809 blocking peptide
Related Product 2: CSF1R pan-specific antibody (Cat. No.: AB-NK234-1)
Related Product 3: CSF1R pan-specific antibody (Cat. No.: AB-NK234-2P)
Related Product 4: CSF1R-pS807+pY809 phosphosite-specific antibody (Cat. No.: AB-PK586)
Related Product 5: CSF1R-pY699 phosphosite-specific antibody (Cat. No.: AB-PK587)
Related Product 6: CSF1R -1 pan-specific antibody (Cat. No.: AB-NK234-3)
Related Product 7: CSF1R -2 pan-specific antibody (Cat. No.: AB-NK234-2)
Related Product 8: CSF1R -3 pan-specific antibody (Cat. No.: AB-NK234-4)
Scientific Background: CSF1R (Fms) is a member of the TK group of protein kinases in the PDGFR family. It acts as a membrane receptor for macrophage colony-stimulating factor 1 (CSF1) and IL34, and it functions in the regulation of cell survival, proliferation, and differentiation in hematopoietic precursor cells, specifically monocytes and macrophages. However, this kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord, where it is more moderately expressed. Orthologues of CSF1R are highly conserved in mammals and birds. CSF1R contains a so-called kinase insert domain and is a member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in CSF1R have been associated with providing sustained signals for cell growth and a predisposition to myeloid malignancy. CSF1R has been linked with the development of acute myeloid leukemia, chronic myelomonocytic leukemia, myelodysplasia, hepatocellular carcinomas, breast cancer, and lung squamous cell carcinomas (LSCC). Gain-of-function mutations in the Fms gene are associated with several forms of cancer.
