Product Name:
CSF1R-pY699
Product Number:
ab-pk589
Target Full Name: Macrophage colony-stimulating factor 1 receptor
Target Alias: CD115; C-fms; Colony stimulating factor 1 receptor; CSF-1-R; CSFMR; FMS; Fms proto-oncogene; Kinase CSFR; Macrophage colony stimulating factor I receptor precursor; M-CSFR; FIM2; FIM2; CCDS4302.1; A2VDG3; Q6LEI2; ENSG00000182578
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK587
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y699
Protein UniProt: P07333
Protein SigNET: P07333
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CSF1R (Fms) sequence peptide Cat. No.: PE-04AKD95
Antibody Immunogen Sequence: GVD(pY)KNI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues G696 to I702; In the kinase catallytic domain. This is the major in vivo phosphorylation site in CSF1R.
Production Method: Corresponds to amino acid residues G696 to I702; In the kinase catallytic domain. This is the major in vivo phosphorylation site in CSF1R.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 and Jurkat cells. Very strong immunoreactivity with immunogen peptide on dot blots. Medium-strong immunoreactivity with recombinant human CDF1R on protein dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Medium immunoreactivity on protein dot blots with recombinant human Ftt3 and Kit. No significant cross-reactive proteins detected in Jurkat and HeLa cells. Strongly cross-reactive 45 and 50 kDa proteins were detected in A431 cells, and a 75 kDa cross-reactive protein was evident in sea star oocytes.
Related Product 1: CSF1R-pY699 blocking peptide
Related Product 2: CSF1R pan-specific antibody (Cat. No.: AB-NK234-1)
Related Product 3: CSF1R pan-specific antibody (Cat. No.: AB-NK234-2P)
Related Product 4: CSF1R-pS807+pY809 phosphosite-specific antibody (Cat. No.: AB-PK586)
Related Product 5: CSF1R-pY809 phosphosite-specific antibody (Cat. No.: AB-PK588)
Related Product 6: CSF1R -1 pan-specific antibody (Cat. No.: AB-NK234-3)
Related Product 7: CSF1R -2 pan-specific antibody (Cat. No.: AB-NK234-2)
Related Product 8: CSF1R -3 pan-specific antibody (Cat. No.: AB-NK234-4)
Scientific Background: CSF1R (Fms) is a member of the TK group of protein kinases in the PDGFR family. It acts as a membrane receptor for macrophage colony-stimulating factor 1 (CSF1) and IL34, and it functions in the regulation of cell survival, proliferation, and differentiation in hematopoietic precursor cells, specifically monocytes and macrophages. However, this kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord, where it is more moderately expressed. Orthologues of CSF1R are highly conserved in mammals and birds. CSF1R contains a so-called kinase insert domain and is a member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in CSF1R have been associated with providing sustained signals for cell growth and a predisposition to myeloid malignancy. CSF1R has been linked with the development of acute myeloid leukemia, chronic myelomonocytic leukemia, myelodysplasia, hepatocellular carcinomas, breast cancer, and lung squamous cell carcinomas (LSCC). Gain-of-function mutations in the Fms gene are associated with several forms of cancer.
