Product Name:
Cot-pS334
Product Number:
ab-pk587
Target Full Name: Osaka thyroid oncogene protein-serine kinase (Tpl2); Mitogen-activated protein kinase kinase kinase 8
Target Alias: FLJ10486; M3K8; MAP3K8; Tpl2; TPL2; TPL-2; tumour progression locus 2; Cot; ESTF; EST; Tpl-2; c-Cot; ENSG00000107968
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK585
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S334
Protein UniProt: P41279
Protein SigNET: P41279
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Cot (MAP3K8, TPL2) sequence peptide Cat. No.: PE-04AKB90
Antibody Immunogen Sequence: YPR(pS)AYP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Y331 to P337; In the kinase catalytic subdomain X region.
Production Method: Corresponds to amino acid residues Y331 to P337; In the kinase catalytic subdomain X region.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-weak immunoreactivity of a target-sized protein by Western blotting in A431 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 52-57 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells and in sea star oocytes.
Related Product 1: Cot-pS334 blocking peptide
Related Product 2: Cot pan-specific antibody (Cat. No.: AB-NK042)
Related Product 3: Cot-CT (Cot-2) pan-specific antibody (Cat. No.: AB-NK042-2)
Related Product 4: Cot (Cot-1) pan-specific antibody (Cat. No.: AB-NK042-1)
Scientific Background: Cot (TPL2, MAP3K8) is a protein-serine/threonine kinase of the STE group and STE-Unique family. It is essential in the TLR4 (recognizing LPS) MAPK/ERK-mediated response in the production of pro-inflammatory cytokines. Catalytic activity of Cot is lost with the K167R, or D270A mutations. IL-1 stimulated activity is decreased with S62A. This mutation will also inhibit IL-1 stimulated phosphorylation of T290 along with MEK phosphorylation when associated with A400. Full inhibition of IL-1 induced MEK phosphorylation and partial inhibition of autophosphorylation can occur with a T290A mutation. Partial inhibition of MEK phosphorylation and autophosphorylation has occurred with a T290A or T290E mutation. Partial impairment of MEK phosphorylation induced by IL-1 has been observed with a S400A mutation. When Cot is associated with A62, a complete loss of T290 and MEK phosphorylation occurs with a S400A mutation.
