Product Name:
CLK1-pS337
Product Number:
ab-pk585
Target Full Name: Dual specificity protein kinase CLK1
Target Alias: CDC like kinase 1; CDC-like kinase 1; CLK
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK583
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S337
Protein UniProt: P49759
Protein SigNET: P49759
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CLK1 sequence peptide Cat. No.: PE-04AKA95
Antibody Immunogen Sequence: EHH(pS)TLV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E334 to V340; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues E334 to V340; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 52-57 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and Jurkat cells and in sea star oocytes, except for a weak 78 kDa protein in Jurkat cells.
Related Product 1: CLK1-pS337 blocking peptide
Related Product 2: CLK1-pS337+pT338 phosphosite-specific antibody (Cat. No.: AB-PK584)
Related Product 3: CLKSubtide - CLK2 protein kinase substrate peptide
Related Product 4: CLKSelectide - CLK3 protein kinase substrate peptide
Scientific Background: CLK1 is a protein-serine/threonine kinase of the CMGC group and CLK family. It is a dual-specificity kinase that phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may involve in regulatory mechanisms of RNA splicing. The phosphorylated serine/arginine-rich (SR) proteins involves in the pre-mRNA processing and releasing through nucleus into nucleoplasm. CLK1, which could phosphorylates the specific SR proteins such as ASF/SF2, may directly regulates the activity and compartmentalization of SR splicing factors. CLK1 interacts with PPIG. This kinase shows high variability in human tissue distribution with the highest levels detected in adrenals, brain, kidney, lung, ovary, pituitary, prostate, spinal cord, testes, thymus, thyroid, trachea, and uterus. Orthologues of CLK1 are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes.
