Product Name:
CK2a-pY255
Product Number:
ab-pk584
Target Full Name: Casein protein-serine kinase 2 alpha/ alpha prime
Target Alias: Casein kinase 2, alpha 1 polypeptide; Casein kinase II, alpha chain; CK II; CK2-alpha1; CKIIA; CSK21; CSNK2A1; Kinase CK2-alpha1;
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK582
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y255
Protein UniProt: P68400
Protein SigNET: P68400
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CK2a1 (CSNK2A1) sequence peptide Cat. No.: PE-04AJZ95
Antibody Immunogen Sequence: EDL(pY)DYI(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E252 to I258; In the last quarter of the kinase catalytic domain. This is the major in vivo phosphorylation site in CK2a.
Production Method: Corresponds to amino acid residues E252 to I258; In the last quarter of the kinase catalytic domain. This is the major in vivo phosphorylation site in CK2a.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-weak immunoreactivity of a target-sized protein by Western blotting in A431 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 44-47 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells and sea star oocytes.
Related Product 1: CK2a-pY255 blocking peptide
Related Product 2: CK2a1-ANT pan-specific antibody (Cat. No.: AB-NK041-2)
Related Product 3: CK2Selectide - CK2a1 (CSNK2A1) protein kinase substrate peptide
Related Product 4: HSP90a (255-268) KinSub - Heat shock protein alpha (K255-E268, human) peptide
Related Product 5: CK2pSelectide - CK2a2 (CSNK2A2) protein kinase substrate peptide
Scientific Background: CK2a1 (CSNK2A1) is a protein-serine/threonine kinase of the Other group and CK2 family. CK2a1 is activated by the CK2-beta regulatory subunit and also by binding p38a MAPK. CK2 is potently inhibited by heparin. It is involved in various cellular processes, such as cell cycle control, apoptosis, transcription, and viral infection. It can phosphorylate human p53 protein a tS392 with presence of UV irradiation, which may be a potential mechanism for p53 activation by UV irradiation. It also phosphorylates the caspases CASP9 and CASP2, and hence negatively regulates apoptosis. CK2a1 levels are often up-regulated by 2-fold in expression in many human tumours.
