Product Name:
Chk1-pS280
Product Number:
ab-pk579
Target Full Name: Checkpoint protein-serine kinase 1
Target Alias: CHEK1; CCDS8459.1; ENSG00000149554
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK577
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S280
Protein UniProt: O14757
Protein SigNET: O14757
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Chk1 (CHEK1) sequence peptide Cat. No.: PE-04AJV95
Antibody Immunogen Sequence: RVT(pS)GGV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R277 to V283; After the kinase catalytic domain in the second half of the kinase
Production Method: Corresponds to amino acid residues R277 to V283; After the kinase catalytic domain in the second half of the kinase
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in HeLa cells with nocodazole overnight treatment. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 54-58 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Nocodazole treatment of HeLa yielded strong increases in 100 as well as 60, 70 and 130 kDa cross-reactive proteins. It is possible that the strong 60 kDa protein is actually the target.
Related Product 1: Chk1-pS280 blocking peptide
Related Product 2: Chk1-pS317 phosphosite-specific antibody (Cat. No.: AB-PK578)
Related Product 3: Chk1-pS345 phosphosite-specific antibody (Cat. No.: AB-PK579)
Related Product 4: Cdc25C (205-225), KinSub - Cdc25C phosphatase (V205-R225, human) peptide; Chktide peptide
Related Product 5: Chk1Selectide - Chk1 (CHEK1) protein kinase substrate peptide
Scientific Background: Chk1 (CHEK1) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is required for checkpoint-mediated cell cycle arrest. It appears to be a tumour suppressor protein (TSP). The active form of the protein kinase normally acts to inhibit tumour cell proliferation. It activates DNA repair (i.e. homologous recombination) by phosphorylating RAD51 or FANCE in response to the presence of damage or unreplicated DNA. It regulates cell cycle progression via several mechanisms: 1) phosphorylation of certain proteins such as CDC25A, CDC25B and CDC25C, promoting their degradation; 2) phosphorylation and activation of p53; 3) phosphorylation and inactivation of TLK1, which consequently prevents TLK1-dependent phosphorylation of chromatin assembly factor ASF1A; 4) phosphorylation of histones and regulating transcription of genes involved in cell-cycle progression regulation; 5) phosphorylation of RB1 to promote its interaction with E2F transcription factors and subsequent cell cycle arrest. It is nhibited by phosphorylation at S280. S345A mutation of Chk1 leads to reduced phosphorylation and impaired activation to nuclear retention after checkpoint activation. Mutations at 372, 376, and 379 amino acids lead to misfolding and induced binding with ubiquitin.
