Product Name:
CDKL5-pY171
Product Number:
ab-pk578
Target Full Name: Cyclin-dependent kinase-like 5; Serine-threonine-protein kinase 9
Target Alias: Cyclin-dependent kinase-like 5; Kinase CDKL5; STK9
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK576
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y171
Protein UniProt: O76039
Protein SigNET: O76039
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDKL5 (STK9) sequence peptide Cat. No.: PE-04ACN90
Antibody Immunogen Sequence: YTE(pY)VAT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Y168 to T174; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues Y168 to T174; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110-120 kDa.
Related Product 1: CDKL5-pY171 blocking peptide
Scientific Background: CDKL5 is a protein-serine/threonine kinase of the CMGC group and CDKL family. It is likely that phosphorylation at Y24 is inhibitory and phosphorylation at Y171 is required for catalytic activity based on homology with other CDK's. It has been reported to phosphorylate MECP2. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. It has also been linked with Rett Syndrome, which is a neuro-development disorder affecting psychomotor development, autistic like features, seizures, and slow head growth. Rett Syndrome is also associated with neuroblastomas.
