Product Name:
CDK9-pS347
Product Number:
ab-pk576
Target Full Name: Cyclin-dependent protein-serine kinase 9
Target Alias: C-2K; CDC2L4; Cell division cycle 2-like protein kinase 4; Cyclin-dependent kinase 9; Kinase CDK9; PITALRE; TAK
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK574
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S347
Protein UniProt: P50750
Protein SigNET: P50750
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK9 sequence peptide Cat. No.: PE-04AJT99
Antibody Immunogen Sequence: RKG(pS)QIT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R344 to T350; Near the C-terminus of the kinase after the catalytic domain
Production Method: Corresponds to amino acid residues R344 to T350; Near the C-terminus of the kinase after the catalytic domain
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa and Jurkat cells and sea star oocytes., except for a strong ~90 kDa cross-reactive protein in Jurkat cells cultured in the presence of FBS.
Related Product 1: CDK9-pS347 blocking peptide
Related Product 2: CDK9-pT186 phosphosite-specific antibody (Cat. No.: AB-PK575)
Related Product 3: CDK7/9Subtide - CDK7 protein kinase substrate peptide
Related Product 4: RNA polymerase II-CT KinSub - heptapeptide repeat from C-terminus peptide; CDKtide protein kinase substrate peptide
Scientific Background: CDK9 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is a protein kinase that is involved in chromatin binding and transcription regulator region DNA binding. Phosphorylation at S175 increases phosphotransferase activity.
