Product Name:
CDKL5-pY171
Product Number:
ab-pk576
Target Full Name: Cyclin-dependent kinase-like 5; Serine-threonine-protein kinase 9
Target Alias: Cyclin-dependent kinase-like 5; Kinase CDKL5; STK9
Product Type Specific: CDKL5 protein kinase phosphosite-specific antibody
Antibody Code: PK576
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y171
Protein UniProt: O76039
Protein SigNET: CDKL5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDKL5 (STK9) sequence peptide Cat. No.: PE-04ACN90
Antibody Immunogen Sequence: YTE(pY)VAT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues Y168 to T174. Y171 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major in vivo phosphorylation site in CDKL5 (≥548 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). CDKL5 is known to be phosphorylated at this site in vitro by CDKL5 (STK9).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110-120 kDa.
Related Product 1: CDKL5 (168-174) pY171 antibody blocking peptide (Cat. No.: PE-04ACN90)
Related Product 2: CDKL2 expression antibody (Cat. No.: AB-NK261-1)
Related Product 3: CDKL3 expression antibody (Cat. No.: AB-NK262-1)
Scientific Background: CDKL5 ((Cyclin-Dependent Kinase-Like 5)) is a protein-serine/threonine kinase of the CMGC group and CDKL family. There are at least five different isoforms resulting from the gene that vary in length and tissue distribution. It is predominantly expressed in the brain, particularly in neurons. CDKL5 is crucial for normal brain development, synapse function, neuronal migration, outgrowth of axons, and dendritic morphogenesis.. It acts by phosphorylating target proteins, regulating dendritic spine structure, and cell signaling. Mutations in the CDKL5 gene cause a severe neurodevelopmental disorder (CDKL5 deficiency disorder) featuring infantile spasms, early-onset epilepsy, and developmental delay. It is likely that phosphorylation at Tyr-24 is inhibitory and phosphorylation at Tyr-171 is required for catalytic activity based on homology with other CDK's. It has been reported to phosphorylate MECP2. It is located at excitatory synapses and within the nucleus, supporting synaptic activity, neuron growth, and RNA splicing. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. It has also been linked with Rett Syndrome, which is a neuro-development disorder affecting psychomotor development, autistic like features, seizures, and slow head growth. Rett Syndrome is also associated with neuroblastomas. Defects in CDKL5 also cause severe intellectual disability, global development delay, and limited or absent speech.This description may include information annotated by UniProt and/or Google AI.