Product Name:
CDK6-pY24
Product Number:
ab-pk574
Target Full Name: Cyclin-dependent protein-serine kinase 6
Target Alias: CRK2; Cyclin-dependent kinase 6; Kinase CDK6; PLSTIRE; Serine/threonine protein kinase PLSTIRE; MGC59692; CCDS5628.1; ENSG00000105810
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK572
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y24
Protein UniProt: Q00534
Protein SigNET: Q00534
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK6 sequence peptide Cat. No.: PE-04AJQ99
Antibody Immunogen Sequence: EGA(pY)GKV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E21 to V27; In the protein kinase catalytic domain in subdomain I.
Production Method: Corresponds to amino acid residues E21 to V27; In the protein kinase catalytic domain in subdomain I.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 34-38 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HepG2, HeLa and MCF7 cells and in sea star oocytes, except for medium ~75 kDa cross-reactive protein in HeLa cells.
Related Product 1: CDK6-pY24 blocking peptide
Related Product 2: CDK6-pY13 phosphosite-specific antibody (Cat. No.: AB-PK571)
Related Product 3: CDK4/6Selectide - CDK4 protein kinase substrate peptide
Related Product 4: RNA polymerase II-CT KinSub - heptapeptide repeat from C-terminus peptide; CDKtide protein kinase substrate peptide
Scientific Background: CDK6 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is involved in cell cycle regulation and differentiation, which promotes G1/S transition. Phosphorylation at T177 increases phosphotransferase activity. Germline mutations in CDK6 are hypothesized to lead to a predisposition to melanoma. A197T mutation is found in family with autosomal recessive primary microcephaly-12 (MCPH12).
