Product Name:
CDK5-pY15
Product Number:
ab-pk572
Target Full Name: Cyclin-dependent protein-serine kinase 5
Target Alias: Cell division protein kinase 5; CRK6; PSSALRE; TAU protein kinase II; TPKII; ENSG00000164885
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK570
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y15
Protein UniProt: Q00535
Protein SigNET: Q00535
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK5 sequence peptide Cat. No.: PE-04AJO95
Antibody Immunogen Sequence: EGT(pY)GTV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E12 to V18; In the protein kinase catalytic domain in subdomain I.
Production Method: Corresponds to amino acid residues E12 to V18; In the protein kinase catalytic domain in subdomain I.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 29-35 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells and sea star oocytes.
Related Product 1: CDK5-pY15 blocking peptide
Related Product 2: CDK5Selectide - CDK5 protein kinase substrate peptide
Scientific Background: CDK5 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is required for cell cycle arrest in neuronal cells. It is activated by binding p35 protein. Phosphorylation at S159 increases phosphotransferase activity. Phosphorylation at T14 and Y15 inhibits phosphotransferase activity. CDK5 has been observed to be proline-directed and to phosphorylate SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. CDK5 has been implicated in Aneurysmal bone cyst, which is a bone cancer that will affect bone, endothelial tissue, and bone marrow.
