Product Name:
CDK5-pY15
Product Number:
ab-pk570
Target Full Name: Cyclin-dependent protein-serine kinase 5
Target Alias: Cell division protein kinase 5; CRK6; PSSALRE; TAU protein kinase II; TPKII; ENSG00000164885
Product Type Specific: CDK5 protein kinase phosphosite-specific antibody
Antibody Code: PK570
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y15
Protein UniProt: Q00535
Protein SigNET: CDK5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK5 sequence peptide Cat. No.: PE-04AJO95
Antibody Immunogen Sequence: EGT(pY)GTV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E12 to V18; in the protein kinase catalytic domain in subdomain I.Y15 phosphorylation inhibits phosphotransferase activity. This is the major in vivo phosphorylation site in CDK5 (≥915 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). CDK5 is known to be phosphorylated at this site in vitro by Abl1, EphA4, Fyn, and TrkA (NTRK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Chicken |Frog | Zebra fish | Fruit fly | Honey bee |Corn | Thale cress.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 29-35 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 cells and sea star oocytes.
Related Product 1: CDK5 (12-18) pY15 antibody blocking peptide (Cat. No.: PE-04AJO95)
Related Product 2: CDK5Selectide kinase substrate peptide (Cat. No.: PE-01BGQ95)
Related Product 3: CDK5 expression antibody (Cat. No.: AB-NK028-2)
Related Product 4: CDK5 S159 phosphorylation antibody (Cat. No.: AB-PK899)
Scientific Background: CDK5 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is required for cell cycle arrest in neuronal cells. It regulates neurite outgrowth, neuronal differentiation, and synaptic vesicle recycling. CDK5 is involved in cell motility, survival, apoptosis, and angiogenesis in non-neuronal tissues. It is activated by binding p39 and it truncated p35 protein. Phosphorylation at Ser-159 increases phosphotransferase activity. Phosphorylation at Thr-14 and Tyr-15 inhibits phosphotransferase activity. CDK5 has been observed to be proline-directed and to phosphorylate SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. CDK5 has been implicated in Aneurysmal bone cyst, which is a bone cancer that will affect bone, endothelial tissue, and bone marrow. Many neurological disorders appear to include targets for CDK5, such as Tau in Alzheimer's disease. This description may include information annotated by UniProt and/or Google AI.