Product Name:
CDK2-pT160
Product Number:
ab-pk570
Target Full Name: Cyclin-dependent protein-serine kinase 2
Target Alias: Cyclin-dependent kinase 2; Kinase CDK2; p33 protein kinase; p33(CDK2); CCDS8898.1; ENSG00000123374
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK568
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T160
Protein UniProt: P24941
Protein SigNET: P24941
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK2 sequence peptide Cat. No.: PE-04ADY95
Antibody Immunogen Sequence: RTY(pT)HEV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R157 to V163; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues R157 to V163; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 33-37 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Medium to strong cross-reactivity with a ~78 kDa protein in HeLa cells, Jurkat cells, and sea star oocytes. The 78 kDa protein band shifts up with phorbol ester treatment of serum-starved HeLa and Jurkat cells or sea star oocyte maturation.
Related Product 1: CDK2-pT160 blocking peptide
Related Product 2: CDK1-3 Selectide - CDK1 (CDC2) protein kinase substrate peptide
Related Product 3: RNA polymerase II-CT KinSub - heptapeptide repeat from C-terminus peptide; CDKtide protein kinase substrate peptide
Scientific Background: CDK2 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is essential for meiosis, yet not essential for mitosis, although it is involved in cell cycle control. CDK2 has been observed to phosphorylate CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, and EZH2. Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. Mutations resulting in reduced CAK phosphorylation at the T160 activating phosphosite include K9F, and K88E + K89V, while a L166R mutation results in reduced CAK phosphorylation and lower protein-serine/threonine phosphotransferase activity. Phosphorylation at Y179 increases phosphotransferase activity and interaction with CCNA2 (Cyclin A2). CCNA2 stimulates the catalytic activity of CDK2. Phosphorylation at T14 and Y15 inhibits phosphotransferase activity.
