Product Name:
CDK12-pS383+pS385
Product Number:
ab-pk568
Target Full Name: Cell division protein kinase 12; Cell division cycle 2-related protein kinase 7
Target Alias: CDK12; CD2L7; CDC2 related protein kinase 7; CDC2- related protein kinase 7; Cdc2-related kinase, arginine/serine-rich; CDC2-related protein kinase 7; CRKR; CRKRS; CrkRS; KIAA0904
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK566
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S383+S385
Protein UniProt: Q9NYV4
Protein SigNET: Q9NYV4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK12 (Cdc2L7) sequence peptide Cat. No.: PE-04ALR99
Antibody Immunogen Sequence: RHS(pS)I(pS)PVR(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R380 to R388; In the first quarter of the protein. These are This is the major in vivo phosphorylation sites in CDK12.
Production Method: Corresponds to amino acid residues R380 to R388; In the first quarter of the protein. These are This is the major in vivo phosphorylation sites in CDK12.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 200-220 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in sea star oocytes, and only one strong ~76 kDa cross-reactive protein in HeLa cells.
Related Product 1: CDK12-pS383+pS385 blocking peptide
Related Product 2: CDK12-pT893 phosphosite-specific antibody (Cat. No.: AB-PK567)
Scientific Background: CDK12 (CRK7) is a protein-serine/threonine kinase of the CMGC group and CDK family. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
