Product Name:
CDK2-pT160
Product Number:
ab-pk568
Target Full Name: Cyclin-dependent protein-serine kinase 2
Target Alias: Cyclin-dependent kinase 2; Kinase CDK2; p33 protein kinase; p33(CDK2); CCDS8898.1; ENSG00000123374
Product Type Specific: CDK2 protein kinase phosphosite-specific antibody
Antibody Code: PK568
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T160
Protein UniProt: P24941
Protein SigNET: CDK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK2 sequence peptide Cat. No.: PE-04ADY95
Antibody Immunogen Sequence: RTY(pT)HEV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R157 to V163; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T160 phosphorylation stimulates phosphotransferase activity. This is one of the major in vivo phosphorylation sites in CDK2 (≥267 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). CDK2 is known to be phosphorylated at this site in vitro by CCRK (CDK20), CDK2, CDK7, ERK1 (MAPK3), and ERK2 (MAPK1).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Frog | Zebra fish | Fruit fly | Honey bee | Sea urchin.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 33-37 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Medium to strong cross-reactivity with a ~78 kDa protein in HeLa cells, Jurkat cells, and sea star oocytes. The 78 kDa protein band shifts up with phorbol ester treatment of serum-starved HeLa and Jurkat cells or sea star oocyte maturation.
Related Product 1: CDK2 (157-163) pT160 antibody blocking peptide (Cat. No.: PE-04ADY95)
Related Product 2: CDK1-3 Selectide kinase substrate peptide (Cat. No.: PE-01BGO99)
Related Product 3: RNA polymerase II-CT KinSub - heptapeptide repeat from C-terminus peptide; CDKtide protein kinase substrate peptide
Related Product 4: CDC2 expression antibody (Cat. No.: AB-NK025-4)
Related Product 5: CDK1 expression antibody (Cat. No.: AB-NK025-7)
Related Product 6: CDK1 T14 phosphorylation antibody (Cat. No.: AB-PK559)
Related Product 7: CDK1 T14+Y15 phosphorylation antibody (Cat. No.: AB-PK560)
Related Product 8: CDK1 Y19 phosphorylation antibody (Cat. No.: AB-PK563)
Related Product 9: CDK1 T161 phosphorylation antibody (Cat. No.: AB-PK561)
Scientific Background: CDK2 is a 34 kDa protein-serine/threonine kinase of the CMGC group and CDK family. It is essential for meiosis, yet not essential for mitosis, although it is involved in cell cycle control. It specifically managing the transition from G1 to S phase and facilitating DNA synthesis. Through its PSTAIRE motif (Pro-45 to Glu-51), it forms active complexes with cyclin (CCN) E (early stage, G1/S phases) and cyclin A (late stage, S phase) to drive cell division and centrosome duplication. It also interacts with cyclins B1, B3, and D. CDK2 has been observed to phosphorylate CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, and EZH2. The phosphotransferase activity of CDK2 is maximal during S phase and G2. Mutations resulting in reduced CAK phosphorylation at the Thr-160 activating phosphosite include K9F, and K88E + K89V, while a L166R mutation results in reduced CAK phosphorylation and lower protein-serine/threonine phosphotransferase activity. Phosphorylation at Tyr-179 increases phosphotransferase activity and interaction with CCNA2. CCNA2 stimulates the catalytic activity of CDK2. Phosphorylation at Thr-14 and Tyr-15 inhibits phosphotransferase activity. CDK2 interacts with inhibitors like p21Cip1 and p27Kip1, which can stop the cell cycle at DNA damage checkpoints. It is ubiquitously expressed in proliferating cells and is found in the nucleus and cytoplasm. This description may include information annotated by UniProt and/or Google AI.