Product Name:
CDK10-pT196
Product Number:
ab-pk566
Target Full Name: Cyclin-dependent protein-serine kinase 10 [PISSLRE]
Target Alias: PISSLRE; ENSG00000185324
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK564
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T196
Protein UniProt: Q15131
Protein SigNET: Q15131
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK10 (PISSLRE) sequence peptide Cat. No.: PE-04AJI90
Antibody Immunogen Sequence: KPM(pT)PKV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K193 to V199; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues K193 to V199; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in maturing sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-40 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in sea star oocytes.
Related Product 1: CDK10-pT196 blocking peptide
Related Product 2: CDK10-ANT pan-specific antibody (Cat. No.: AB-NK033-2)
Related Product 3: CDK10-CT pan-specific antibody (Cat. No.: AB-NK033-3)
Scientific Background: CDK10 (PISSLRE) is a protein-serine/threonine kinase of the CMGC group and CDK family. It is a protein kinase that mediates phosphorylation and degradation of ETS2 (a transcription factor). CDK10 appears to be a tumour requiring protein (TRP). It displays one of the lowest rates of mutation in human tumours. The near absence of reported somatic mutations in this protein indicates that it may function as a tumour requiring protein (TRP) in many human tumours.
