Product Name:
CDK11A-pT583
Product Number:
ab-pk565
Target Full Name: Cell division cycle 2-like 2 protein kinase
Target Alias: CDK19; CD2L6; CDC2L6; CDK8-like; CDK8-like cyclin-dependent kinase; CDK8-like cyclin-dependent kinase 11; Cell division cycle 2-like 6; Cyclin-dependent kinase (CDC2-like) 11; Death-preventing kinase; KIAA1028; CDC2L1; CDK11A; CDK11-p110; CDK11-p46; CDK11-p58; CLK-1; FLJ59152; p58; p58CDC2L1; p58CLK-1; PITSLRE; PK58; Q9UQ88; A4VCI5; Q5QPQ9; Q6P5Y5
Product Type Specific: CDK11A protein kinase phosphosite-specific antibody
Antibody Code: PK565
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T583
Protein UniProt: Q9UQ88
Protein SigNET: CDK11A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK11A (Cdc2L2; PITSLRE) sequence peptide Cat. No.: PE-04AJJ95
Antibody Immunogen Sequence: KAY(pT)PVV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K580 to V586. T583 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major in vivo phosphorylation sites in CDK11a (≥49 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in sea star oocytes and weaker in Jurkat cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Honey bee .
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-100 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactivities detected in Jurkat, Hela, Jurkat and MCF7 cells, but insulin increases 75 kDa in MCF7 cells
Related Product 1: CDK11 (580-586) pT583 antibody blocking peptide (Cat. No.: PE-04AJJ95)
Scientific Background: CDK11 (CDK19, CD2L6; PITSLRE) is a cyclin-dependent, protein-serine/threonine kinase of the CMGC group and CDK family. It is a multifunctional kinase essential for cell cycle progression, pre-mRNA splicing, and transcription, specifically of histone genes. It exists in two main isoforms, a full-length CDK11p110 and a smaller, mitosis-specific CDK11p58 generated by internal ribosome entry site (IRES) translation that facilitates mitotic centrosome maturation. Elevated CDK11 is linked to cancer proliferation. CDK11p110 is a component of Mediator coactivator complex, which is required for transcriptional activation. The p58 isoform is active during the G2/M phase, crucial for centrosome maturation and proper sister chromatid cohesion. CDK11, alongside Cyclin L1/L2 and the activator SAP30BP, acts as a kinase that phosphorylates RNA polymerase II (CTD) and spliceosomal components (SF3B1), aiding transcription and splicing. The highest detected expression was in corpus callosum, followed by kidney and ovary. CDK11 is often overexpressed in various cancers, including ovarian, breast, and esophageal cancer, driving cell proliferation. Inhibition of CDK11 causes cell cycle dysfunction, mitotic arrest, and apoptosis in tumour cells. This description may include information annotated by UniProt and/or Google AI.