Product Name:
CDK1-pT14+pY15
Product Number:
ab-pk560
Target Full Name: Cyclin-dependent protein-serine kinase 1; Cell division control protein 2 homologue
Target Alias: Cdc2; CDC28; CDC28A; CDC2A; Cell division control protein 2; Cell division cycle 2, G1 to S and G2 to M; Cyclin-dependent kinase 1; P34 protein kinase; Kinase Cdc2; MPF; DKFZp686L20222; MGC111195; ENSG00000170312
Product Type Specific: CDK1 protein kinase phosphosite-specific antibody
Antibody Code: PK560
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T14+Y15
Protein UniProt: P06493
Protein SigNET: CDK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDK1 (CDC2) sequence peptide Cat. No.: PE-04ACM99
Antibody Immunogen Sequence: EG(pT)(pY)GVV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E12 to V18. T14 and Y15 phosphorylation inhibits phosphotransferase activity. These phosphosites are located in the kinase subdomain I region, which is involved in binding the phosphates in the ATP substrate. Phosphorylation regulates cell cycle progression. These are two of the major in vivo phosphorylation sites in CDK1 (≥1823 and ≥3976 reports, respectively, by mass spectrometry). CDK1 is known to be phosphorylated in vitro at T14 by MYT1 (PKMYT1), and at Y15 by CDK1 (CDC2), Chk1 (CHEK1), MYT1 (PKMYT1), Src, and Wee1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity of a target-sized protein by Western blotting in immmature sea star oocytes. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Nematode worm | Sea urchin | Corn | Thale Cress | Budding yeast.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 30-35 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: In HEK-293 cells, phenylarsine oxide (PAO) decreases 50 kDa + 30 kDa proteins; in Jurkat cells, phenylarsine oxide (PAO) increases 15 kDa protein and very weakly many other proteins.
Related Product 1: CDK1 (12-18) pT14+pY15 antibody blocking peptide (Cat. No.: PE-04ACM99)
Related Product 2: CDK1-3 Selectide kinase substrate peptide (Cat. No.: PE-01BGO99)
Related Product 3: CDC2 expression antibody (Cat. No.: AB-NK025-4)
Related Product 4: CDK1 expression antibody (Cat. No.: AB-NK025-7)
Related Product 5: CDK1 T14 phosphorylation antibody (Cat. No.: AB-PK559)
Related Product 6: CDK1 Y19 phosphorylation antibody (Cat. No.: AB-PK563)
Related Product 7: CDK1 T161 phosphorylation antibody (Cat. No.: AB-PK561)
Related Product 8: CDK2 T160 phosphorylation antibody (Cat. No.: AB-PK568)
Scientific Background: CDK1 (CDC2) is a 34 kDa protein-serine/threonine kinase of the CMGC group and CDK family. It plays an essential role in cell cycle control in eukaryotic cells by regulating the centrosome cycle, mitotic onset, G2-M phase transition, G1 progression, and G1-S phase transition through an association with various interphase cyclin proteins. Beyond mitosis, CDK1 regulates Golgi remodeling, vesicle transport, and is implicated in DNA damage checkpoints. Phosphorylation events at Thr-14 or Tyr-15 on CDK1 are inactivating, while phosphorylation at Thr-161 is stimulatory. It is activated by binding to cyclin B1, followed by activation via CDC25 phosphatase, which removes inhibitory phosphorylation from WEE1/MYT1 kinases. CDK1 phosphorylates over 200 proteins to trigger mitotic events like centrosome separation, nuclear envelope breakdown, and chromosome condensation. CDK1 is found in the nucleus, cytoplasm, centrosomes, and mitotic spindle. Human CDK1 shares approximately 63% amino-acid identity with yeast, allowing it to function across species. Gain-of-function mutations in the CDK1 gene have been linked to several forms of cancer, indicating an oncogenic role for the CDK1 protein. Cells transformed with the oncogene MYC undergo apoptosis when treated with small-molecule CDK1 inhibitors. Elevated expression of CDK1 has been reported as a diagnostic marker for cancer progression in esophageal adenocarcinoma, potentially reflecting the role of the CDK1 protein in tumorigenesis. CDK1 expression can be used as a prognostic indicator for early breast cancer. For example, breast cancer tumours with high expression of CDK1 are correlated with a significantly lower 5-year patient survival rate (66. 9%) than tumours that have low levels of CDK1 expression (84. 2%). This description may include information annotated by UniProt and/or Google AI.