Product Name:
CaMKK1-pS74
Product Number:
ab-pk558
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase kinase
Target Alias: Calcium/calmodulin-dependent protein kinase 1 alpha, isoform a; Calcium/calmodulin-dependent protein kinase kinase 1, alpha; CaMKK alpha protein; CaMKK1.; CaMKKA; DKFZP761M0423; KKCC1; MGC34095
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK557
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S74
Protein UniProt: Q8N5S9
Protein SigNET: Q8N5S9
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMKK1 (CaMKK) sequence peptide Cat. No.: PE-04AJD99
Antibody Immunogen Sequence: RKL(pS)LQE(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R71 to E77; In the N-terminal quarter of the protein located before the kinase catalytic domain. On the This is the major in vivo phosphorylation sites in CaMMK1.
Production Method: Corresponds to amino acid residues R71 to E77; In the N-terminal quarter of the protein located before the kinase catalytic domain. On the This is the major in vivo phosphorylation sites in CaMMK1.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.25 / 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Weak 37 kDa cross-reactivity detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells, and insulin-treated MCF7 cells, when these cells were directly homogenized into SDS-PAGE sample buffer.
Related Product 1: CaMKK1-pS74 blocking peptide
Related Product 2: CaMKK1 (CaMKK1-1) pan-specific antibody (Cat. No.: AB-NK022)
Scientific Background: CaMKK1 is a protein-serine/threonine kinase of the Other group and CaMKK family. It is a calcium/calmodulin-activated protein kinase that phosphorylates and activates CaMK1, CaMK1d, CaMK1g, CaMK4 and Akt1. Binding of calmodulin may releave intrasteric autoinhibition. It is partially inhibited upon phosphorylation by PRCAKA/PKA
