Product Name:
CaMK2a-pT286
Product Number:
ab-pk556
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 2 alpha
Target Alias: Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II alpha-B subunit; Calcium/calmodulin-dependent protein kinase type II alpha chain; CaM kinase II alpha subunit; CaMK2-alpha; CaMKA; CaMK-II alpha subunit; CaMKII-alpha; CaMKIINalpha; Kinase CaMK2-alpha;CaM-kinase II alpha chain; EC 2.7.11.17; KCC2A; KIAA0968
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK555
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T286
Protein UniProt: Q9UQM7
Protein SigNET: Q9UQM7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK2a sequence peptide Cat. No.: PE-04AJB90
Antibody Immunogen Sequence: HRQE(pT)VDC
Antibody Immunogen Description: Corresponds to amino acid residues H282 to C289; In the region between the kinase catalytic domain and the CamKII-AD domains. The major site of in vivo phosphorylation of CaMK2a.
Production Method: Corresponds to amino acid residues H282 to C289; In the region between the kinase catalytic domain and the CamKII-AD domains. The major site of in vivo phosphorylation of CaMK2a.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-60 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Weak cross-reactive proteins of ~36 and ~75 kDa detected in HeLa cells.
Related Product 1: CaMK2a-pT286 blocking peptide
Related Product 2: CaMKII (Alpha-Specific) pan-specific antibody (Cat. No.: AB-NK302-1)
Related Product 3: CaMKII pT286/pT287 phosphosite-specific antibody (Cat. No.: AB-NK302-2)
Related Product 4: Autocamtide-2 peptide - Autocamtide 2 CaMK2-derived peptide substrate
Related Product 5: CAMK2Subtide - CaMK2a protein kinase substrate peptide
Related Product 6: Syntide-2 KinSub - Syntide-2 protein kinase substrate peptide
Scientific Background: CaMK2a is a protein-serine/threonine kinase of the CaMK group and CaMK2 family. It is a calcium/calmodulin-activated protein kinase involved in neurotransmitter release and long term potentiation in neurons. Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state
