Product Name:
CaMK4-pT200
Product Number:
ab-pk556
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 4
Target Alias: Brain Ca++-calmodulin-dependent protein kinase type IV; Calcium/calmodulin-dependent protein kinase IV; Calcium/calmodulin-dependent protein kinase type IV catalytic chain; Calspermin; CAM kinase- GR; CAM kinase IV; CAM kinase-GR; CaMK IV; CaMK4; Kinase CaMK4; CaMK-GR; CaMKIV; KCC4 CaMK IV; MGC36771; CCDS4103.1; ENSG00000152495
Product Type Specific: CaMK4 protein kinase phosphosite-specific antibody
Antibody Code: PK556
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T200
Protein UniProt: Q16566
Protein SigNET: CaMK4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK4 (CaMPK4) sequence peptide Cat. No.: PE-04AJC90
Antibody Immunogen Sequence: LMK(pT)VCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L197 to G203; in the protein kinase catalytic domain. T200 phosphorylation stimulates phosphotransferase activity and regulates transcription. This is one of the major in vivo phosphorylation sites in CaMK4 (≥13 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). CaMK4 is known to be phosphorylated at this site in vitro by CaMKK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Rat | Mouse | Platypus.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: One medium cross-reactive protein of ~110 kDa detected in HEK-293 cells and more weakly in HeLa cells. This antibody cross-reacts with several phosphoproteins in maturing, germinal vesicle positive sea star oocytes.
Related Product 1: CAMK4 (197-203) pT200 antibody blocking peptide (Cat. No.: PE-04AJC90)
Related Product 2: Autocamtide-2 peptide kinase substrate peptide (Cat. No.: PE-01ACM95)
Related Product 3: https://kinexus-ca.myshopify.com/products/AB-NK021-1
Related Product 4: https://kinexus-ca.myshopify.com/products/AB-NK021-1
Scientific Background: CaMK4 is a protein-serine/threonine kinase of the CaMK group and CaMK1 family. It is a calcium/calmodulin-dependent protein kinase. This kinase is monomeric in structure and occurs in two isoforms generated by alternative splcing from the same gene. It is implicated in immune response, inflammation, memory consolidation, positive selection of T lymphocytes, cytokine production, regulation in neurons and male germ cells, survival and differentiation of osteoblasts and dendritic cells. It is highly expressed in rat tissues such as the forebrain, lymphocytes, spleen, testis, and thymus. Immunohistochemical studies have demonstrated a nuclear localization of the transiently expressed CaMK4. The cytoskeletal and perinuclear localization of inducible CaMK4 implies that the kinase may be a modulator of cell shape and/or motility and/or activity of a cytoskeleton-associated nuclear factor. It regulates many transcription activators such as CREB1, and JUN. It can also activate MAPK pathway through phosphorylation of ELK1 and ATF2. Mutations (S12A, S13A, T57A+K58E, K75E, T200A) are mostly result in loss of phosphotransferase activity or loss of activation by the upstream kinases CaMKK1 or CaMKK2. Some mutations (S189A, H309D+M310E+T312D+F320D+N321D+) lead to a constitutively active form. This description may include information annotated by UniProt and/or Google AI.