Product Name:
CaMK1d-pT180
Product Number:
ab-pk555
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 1 delta
Target Alias: CaMK1D; CaMK1-delta; CaM-KI delta; EC 2.7.11.17; KCC1D
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK554
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T180
Protein UniProt: Q8IU85
Protein SigNET: Q8IU85
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK1d sequence peptide Cat. No.: PE-04AJA99
Antibody Immunogen Sequence: VMS(pT)ACG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V177 to G183; In the protein kinase catalytic domain.
Production Method: Corresponds to amino acid residues V177 to G183; In the protein kinase catalytic domain.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-50 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: A weak cross-reactive protein is detected in sorbitol-treated HeLa cells.
Related Product 1: CaMK1d-pT180 blocking peptide
Related Product 2: Autocamtide-2 peptide - Autocamtide 2 CaMK2-derived peptide substrate
Related Product 3: CREB1 (123-135) KinSub - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide
Related Product 4: CREB1 (123-135) KinSub, biotinyl. - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide
Scientific Background: CaMK1d is a protein-serine/threonine kinase of the CaMK group and CaMK1 family. It is activated by Ca2+/calmodulin. The binding of calmodulin is thought to result in a conformational change and can lead to subsequent activation through phosphorylation by CaMKK1 and CaMKK2. At least in part, it can also be activated by CaMKK1 in a calcium-independent manner. It is associated with calcium-triggered CaMKK-CaMK1 signalling cascade and activates CREB- and CREM-BETA-dependent gene transcription. A K52A mutation leads to a loss of phosphotransferase function.
