Product Name:
CaMK2a-pT286
Product Number:
ab-pk555
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 2 alpha
Target Alias: Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II alpha-B subunit; Calcium/calmodulin-dependent protein kinase type II alpha chain; CaM kinase II alpha subunit; CaMK2-alpha; CaMKA; CaMK-II alpha subunit; CaMKII-alpha; CaMKIINalpha; Kinase CaMK2-alpha;CaM-kinase II alpha chain; EC 2.7.11.17; KCC2A; KIAA0968
Product Type Specific: CaMK2a protein kinase phosphosite-specific antibody
Antibody Code: PK555
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T286
Protein UniProt: Q9UQM7
Protein SigNET: CaMK2a
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK2a sequence peptide Cat. No.: PE-04AJB90
Antibody Immunogen Sequence: HRQE(pT)VDC
Antibody Immunogen Description: Corresponds to amino acid residues H282 to C289; in the region between the kinase catalytic domain and the CamKII-AD domains. The major site of in vivo phosphorylation of CaMK2a. T286 phosphorylation stimulates calcium/calmodulin-independent phosphotransferase activity and regulates cell growth, intracellular location, molecular association, including inducing interaction with GIT1 and MAP2, and inhibiting interaction with MBP and neurogranin. This is a major site of in vivo phosphorylation of CaMK2a (≥88 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). CaMK2a is known to be phosphorylated at this site in vitro by CaMK2a, CaMK2b, CaMK2d, and PKCa (PRKCA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-60 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: Weak cross-reactive proteins of ~36 and ~75 kDa detected in HeLa cells.
Related Product 1: CaMK2a (282-289) pT286 antibody blocking peptide (Cat. No.: PE-04AJB90)
Related Product 2: CaMK2a Y230 phosphorylation antibody (Cat. No.: AB-PK875)
Related Product 3: CaMKII (Alpha expression antibody (Cat. No.: AB-NK302-1)
Related Product 4: CaMKII pT286/pT287 T286 phosphorylation antibody (Cat. No.: AB-NK302-2)
Related Product 5: CaMK2b expression antibody (Cat. No.: AB-NK018-3)
Related Product 6: CaMK2g expression antibody (Cat. No.: AB-NK020-1)
Related Product 7: CaMK2d expression antibody (Cat. No.: AB-NK019-3)
Scientific Background: CaMK2a (CaMKII) is a protein-serine/threonine kinase of the CaMK group and CaMK2 family. It is a calcium/calmodulin-activated protein kinase involved in neurotransmitter release and long term potentiation in neurons. It is highly abundant in the brain, particularly at excitatory synapses in the postsynaptic density. It acts as a key mediator of synaptic plasticity, learning, and memory by regulating long-term potentiation (LTP) and activating upon increased calcium levels. Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. It is also involved in regulation of cell cycle progression, T-cell activation, and cardiac function. CaMK2a resides in large multi-oligomeric protein complexes (12 subunits) with other CaMK2 subunits (beta, gamma and delta). Mutations in the CAMK2A gene are linked to neurodevelopmental disorders, specifically autosomal dominant and recessive intellectual disabilities. This description may include information annotated by UniProt and/or Google AI.