Product Name:
CaMK1a-pT177
Product Number:
ab-pk554
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 1 alpha
Target Alias: Calcium/calmodulin-dependent protein kinase I; Calcium/calmodulin-dependent protein kinase type I; CAM kinase I; CAMK1; CaMK1-alpha; CaMKI; KCC1; KCC1A; Kinase CaMK1-alpha
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK553
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T177
Protein UniProt: Q14012
Protein SigNET: Q14012
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK1a (CaMKI) sequence peptide Cat. No.: PE-04AIZ95
Antibody Immunogen Sequence: VLS(pT)ACG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V174 to G180; In the protein kinase catalytic domain.
Production Method: Corresponds to amino acid residues V174 to G180; In the protein kinase catalytic domain.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: No cross-reactivities, but background is high in A431 cells. In HEK-293 has a slightly higher background with FBS treatment, but not with phenylarsine oxide (PAO) treatment.
Related Product 1: CaMK1a-pT177 blocking peptide
Related Product 2: Autocamtide-2 peptide - Autocamtide 2 CaMK2-derived peptide substrate
Related Product 3: CAMK1Subtide - CaMK1a protein kinase substrate peptide
Related Product 4: ZIPtide KinSub - DAPK3 (ZIPK) protein kinase substrate peptide
Scientific Background: CaMK1a is a protein-serine/threonine kinase of the CAMK group and CAMK1 family. Phosphorylation at Thr-177 increases its phosphotransferase activity by approximately 25-fold in a Ca(2+)-CaM dependent manner. Thr-177 is phosphorylated by CAMKK1 or CAMKK2. CaMK1a and CaMKK1 regulate the G(O)-G(1) restriction check point for cell cycle transition in human breast cancer cells. Target substrates for CaMK1a includes CREB1, ATF1, CFTR, MYL9, and SYN1/synapsin I.
