Product Name:
CaMK1d-pT180
Product Number:
ab-pk554
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 1 delta
Target Alias: CaMK1D; CaMK1-delta; CaM-KI delta; EC 2.7.11.17; KCC1D
Product Type Specific: CaMK1d protein kinase phosphosite-specific antibody
Antibody Code: PK554
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T180
Protein UniProt: Q8IU85
Protein SigNET: CaMK1d
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK1d sequence peptide Cat. No.: PE-04AJA99
Antibody Immunogen Sequence: VMS(pT)ACG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V177 to G183; in the protein kinase catalytic domain. T180 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major in vivo phosphorylation site in CaMK1d (≥19 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). CaMK1d is known to be phosphorylated at this site in vitro by CaMKK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-50 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: A weak cross-reactive protein is detected in sorbitol-treated HeLa cells.
Related Product 1: CaMK1d pT180 antibody blocking peptide (Cat. No.: PE-04AJA)
Related Product 2: Autocamtide-2 peptide kinase substrate peptide (Cat. No.: PE-01ACM95)
Related Product 3: CAMK1Subtide - CaMK1a protein kinase substrate peptide
Related Product 4: CaMK1a T177 phosphorylation antibody (Cat. No.: AB-PK553)
Related Product 5: CAMK1a Y235 phosphorylation antibody (Cat. No.: AB-PK874)
Scientific Background: CaMK1d is a protein-serine/threonine kinase of the CaMK group and CaMK1 family. It is activated by Ca2+/calmodulin. The binding of calmodulin is thought to result in a conformational change and can lead to subsequent activation through phosphorylation by CaMKK1 and CaMKK2. At least in part, it can also be activated by CaMKK1 in a calcium-independent manner. It is associated with calcium-triggered CaMKK-CaMK1 signalling cascade and activates CREB- and CREM-BETA-dependent gene transcription. It is highly expressed in polymorphonuclear leukocytes (PMNs). It regulates neutrophil function (granule function, chemotaxis, and respiratory bursts), promotes basal dendritic growth of hippocampal neurons, and is associated with insulin secretion in pancreatic beta-cells and linked to type 2 diabetes risk. CaMK1d also plays a role in the apoptosis of erythroleukemia cells and it has been linked to immune resistance in tumour cells. It features an autoinhibitory domain, a calmodulin-binding region, and a catalytic domain. A K52A mutation leads to a loss of phosphotransferase function. This description may include information annotated by UniProt and/or Google AI.