Product Name:
BUB1B-pS670
Product Number:
ab-pk553
Target Full Name: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Target Alias: BUB1 budding uninhibited by benzimidazoles 1 homologue beta (yeast); HBUBR1; Kinase BubR1; MAD3/BUB1-related protein kinase; MAD3L; SSK1; Bub1A; hBUBR1; BUB1beta; hBUBR1; CCDS10053.1; ENSG00000156970
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK552
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S670
Protein UniProt: O60566
Protein SigNET: O60566
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human BUB1B (Bub1R) sequence peptide Cat. No.: PE-04AIY99
Antibody Immunogen Sequence: KKL(pS)PII(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues K667 to I673; Just before kinase catalytic domain. This is the major in vivo phosphorylation sites.
Production Method: Corresponds to amino acid residues K667 to I673; Just before kinase catalytic domain. This is the major in vivo phosphorylation sites.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in HeLa and T98G cells. Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 115-130 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in T98G cells, but a strong ~80 kDa protein was evident in HeLa cells, and a strong ~70 kDa protein was seen in sea star oocytes.
Related Product 1: BUB1B-pS670 blocking peptide
Scientific Background: BUBR1 (BUB1B) is a protein-serine/threonine kinase of the Other group and BUB family. It is an essential component of the mitotic checkpoint and is required for normal mitosis. It prevents progression of anaphase until all the sister chomatids are connected to spindles by binding to CDC20 to anaphase promoting complex (APC)/cycolsome. It is activated by the kinetochore motor protein CENPE, which increases phosphotransferase activity, and it is also involved in the localization of CENPE. BUBR1 negatively regulates Plk1 phosphotransferase activity in interphase cells, and it suppresses centrosome amplification and triggers apoptosis in polyploid cells that exit aberrantly from mitotic arrest. A K795R mutation inhibits the phosphotransferase activity of BUBR1, but does not abolish its ability to inhibit APC. BUBR1 may be a tumour suppressor protein (TSP). An R36Q mutation is linked with premature chromatid separation trait (PCS), which is an autosomal dominant disease. An R550Q mutation is found in Mosaic variegated aneuploidy syndrome 1 (MVA1), which is a severe developmental disease with characterized mosaic aneuploidies.
