Product Name:
CaMK1a-pT177
Product Number:
ab-pk553
Target Full Name: Calcium/calmodulin-dependent protein-serine kinase 1 alpha
Target Alias: Calcium/calmodulin-dependent protein kinase I; Calcium/calmodulin-dependent protein kinase type I; CAM kinase I; CAMK1; CaMK1-alpha; CaMKI; KCC1; KCC1A; Kinase CaMK1-alpha
Product Type Specific: CaMK1a protein kinase phosphosite-specific antibody
Antibody Code: PK553
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T177
Protein UniProt: Q14012
Protein SigNET: CaMK1a
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CaMK1a (CaMKI) sequence peptide Cat. No.: PE-04AIZ95
Antibody Immunogen Sequence: VLS(pT)ACG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues V174 to G180; in the protein kinase catalytic domain. T177 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major in vivo phosphorylation sites in CaMK1a (≥12 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). CaMK1a is known to be phosphorylated at this site in vitro by CAMKK1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 40-45 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: No cross-reactivities, but background is high in A431 cells. In HEK-293 has a slightly higher background with FBS treatment, but not with phenylarsine oxide (PAO) treatment.
Related Product 1: CaMK1a (174-180) pT177 antibody blocking peptide (Cat. No.: PE-04AIZ95)
Related Product 2: Autocamtide-2 peptide kinase substrate peptide (Cat. No.: PE-01ACM95)
Related Product 3: CAMK1Subtide - CaMK1a protein kinase substrate peptide
Related Product 4: CAMK1a Y235 phosphorylation antibody (Cat. No.: AB-PK874)
Related Product 5: CaMK1d T180 phosphorylation antibody (Cat. No.: AB-PK554)
Scientific Background: CaMK1a is a protein-serine/threonine kinase of the CAMK group and CAMK1 family. It plays a key role in mediating calcium signals to downstream targets, such as the transcription activator HDAC5 or the signalling protein MARK2. Phosphorylation at Thr-177 increases its phosphotransferase activity by approximately 25-fold in a Ca(2+)-CaM dependent manner. Thr-177 is phosphorylated by CAMKK1 or CAMKK2. It is essential for neuronal differentiation, dendritic spine growth, and the creation of long-term potentiation (LTP) in hippocampal neurons. It regulates axonal extension by affecting growth cone motility. It promotes neurite outgrowth, axon extension, and spine formation by activating targets like MARK2 and ARHGEF7. Often found in the cytoplasm and postsynaptic density, with roles in mediating NMDA receptor-induced calcium signalling. Beyond the nervous system, CaMK1a is implicated in cell cycle regulation, actin filament organization, and muscle cell differentiation. CaMK1a and CaMKK1 regulate the G(O)-G(1) restriction check point for cell cycle transition in human breast cancer cells. Other target substrates for CaMK1a includes CREB1, ATF1, CFTR, MYL9, and SYN1/synapsin I. It is activated by calcium/calmodulin, it mediates NMDA receptor-dependent neuronal development, often by phosphorylating ARHGEF7/β-PIX. This description may include information annotated by UniProt and/or Google AI.