Product Name:
BRSK1-pT189
Product Number:
ab-pk550
Target Full Name: BR serine/threonine-protein kinase 1
Target Alias: BR serine/threonine kinase 1; KIAA1811; SAD1; SAD1 kinase; SAD1A
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK549
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T189
Protein UniProt: Q8TDC3
Protein SigNET: Q8TDC3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human BRSK1 sequence peptide Cat. No.: PE-04AIW95
Antibody Immunogen Sequence: LLE(pT)SCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L186 to G192; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues L186 to G192; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in HEK-293 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-90 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactive proteins evident in Jurkat cells, HEK-293 cells and in sea star oocytes.
Related Product 1: BRSK1-pT189 blocking peptide
Related Product 2: BrskSelectide - BRSK1 protein kinase substrate peptide
Related Product 3: BrskSubtide - BRSK2 protein kinase substrate peptide
Scientific Background: BRSK1 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is activated by phosphorylation on T205 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Its substrates include CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Its phosphotransferase activity is lost with the K59A, and G327A mutations. It is involved in the UV-induced DNA damage checkpoint response, likely by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C. A LKB1/LKB1 complex with the STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 phosphorylates and activates BRSK1, but this can be prevented by an inhibitory T189A mutation.
