Product Name:
BRSK1-pT189
Product Number:
ab-pk549
Target Full Name: BR serine/threonine-protein kinase 1
Target Alias: BR serine/threonine kinase 1; KIAA1811; SAD1; SAD1 kinase; SAD1A
Product Type Specific: BRSK1 protein kinase phosphosite-specific antibody
Antibody Code: PK549
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T205
Protein UniProt: Q8TDC3
Protein SigNET: BRSK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human BRSK1 sequence peptide Cat. No.: PE-04AIW95
Antibody Immunogen Sequence: LLE(pT)SCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L186 to G192; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T205 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is a minor in vivo phosphorylation site in BRSK1 (≥1 report by mass spectrometry). BRSK1 is known to be phosphorylated at this site in vitro by BRSK1, CaMK2a, and LKB1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in HEK-293 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 85-90 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactive proteins evident in Jurkat cells, HEK-293 cells and in sea star oocytes.
Related Product 1: BRSK1 (186-192) pT189 antibody blocking peptide (Cat. No.: PE-04AIW95)
Related Product 2: BrskSelectide kinase substrate peptide (Cat. No.: PE-01BGK99)
Related Product 3: BrskSubtide kinase substrate peptide (Cat. No.: PE-01BGL99)
Scientific Background: BRSK1 ((Brain-Selective Kinase 1) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is primarily expressed in the brain, where it plays a critical role in establishing neuronal polarity, axon branching, and synaptic development, as well as testis. It is located in the cytoplasm, nucleoplasm, cell junctions, and synaptic vesicles. It acts downstream of LKB1 (STK11), playing key roles in cell cycle checkpoint signaling (G2/M) and centrosome duplication. It is activated by phosphorylation on Thr-205 by LKB1 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. This can be prevented by an inhibitory T189A mutation. Its substrates include CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Its phosphotransferase activity is lost with the K59A, and G327A mutations. It is involved in the UV-induced DNA damage checkpoint response, likely by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C. Research on mice indicates BRSK1 plays a role in anxiety-like behavior and ethanol-induced GABA release in a sex-dependent manner. BRSK1 plays a role as a tumour suppressor in breast cancer and potentially other cancers, with its inhibition or mutation potentially contributing to cancer development. This description may include information annotated by UniProt and/or Google AI.