Product Name:
Brk-pY342
Product Number:
ab-pk548
Target Full Name: Protein-tyrosine kinase 6
Target Alias: Brk6; PTK6; Tyrosine-protein kinase 6; FLJ42088; CCDS13524.1; Q13882; ENSG00000101213
Product Type Specific: BRK protein kinase phosphosite-specific antibody
Antibody Code: PK548
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y342
Protein UniProt: Q13882
Protein SigNET: Brk
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Brk sequence peptide Cat. No.: PE-04AIV99
Antibody Immunogen Sequence: EDV(pY)LSH(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E339 to H345; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. Y342 phosphorylation stimulates phosphotransferase activity and induces interaction with Brk. Its phosphorylation is commonly observed by mass spectrometry studies (≥48 reports). BRK is known to be phosphorylated at this site in vitro by Brk.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human (Site not well conserved).
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-55 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactive proteins evident in HeLa cells, except for a weak ~38 kDa protein.
Related Product 1: Brk (339-345) pY342 antibody blocking peptide (Cat. No.: PE-04AIV99)
Related Product 2: Brk S446+Y447 phosphorylation antibody (Cat. No.: AB-PK547)
Scientific Background: Brk (PTK6) is a protein-tyrosine kinase of the TK group and Src family. It is a non-receptor kinase that is implicated as a regulator of several intracellular signalling pathways involved in cellular differentiation, epithelial maintenance, and tumorigenesis. The activity of Brk appears to be dependent upon the cell type and intracellular localization of the protein. In non-tumour cells, Brk activity promotes cellular differentiation and apoptosis, while in cancer tissue Brk activity sensitizes cells to growth promoting signals leading to increased cell proliferation, anchorage-independent cell survival, and cell migration/invasion. The nuclear form of the protein is thought to be involved in regulating epithelial growth. The 451-amino acid sequence of the Brk protein resembles that of a Src family kinase, containing a SH3, SH2, and a catalytic domain. However unlike Src kinases, the N-terminal domain of the Brk protein does not contain a myristolyation domain, indicating different regualtory mechanisms. Brk mRNA is expressed at high levels in the colon, small intestine, and prostate as well as at low levels in some fetal tissues. This description may include information annotated by UniProt and/or Google AI.