Product Name:
Bmx-Y40
Product Number:
ab-pk546
Target Full Name: Bone marrow X protein-tyrosine kinase
Target Alias: BMX NON-receptor tyrosine kinase; Bone marrow kinase BMX; Cytoplasmic BMX; Epithelial and endothelial tyrosine kinase; ETK; Kinase Etk; NTK38; Protein tyrosine kinase BMX; PSCTK3; ENSG00000102010
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK545
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y40
Protein UniProt: P51813
Protein SigNET: P51813
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Bmx (Etk) sequence peptide Cat. No.: PE-04AIT95
Antibody Immunogen Sequence: NLS(pY)YEY(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N37 to Y43; In the PH domain domain
Production Method: Corresponds to amino acid residues N37 to Y43; In the PH domain domain
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 75-80 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Cross-reactivities are also seen with 4 other proteins of ~70 to 130 kDa in over night serum-starved Jurkat cells. Weakly cross reacts with phospho-ERK1 in sea star oocytes.
Related Product 1: Bmx-Y40 blocking peptide
Scientific Background: Bmx (Etk) is a protein-tyrosine kinase of the TK group and Tec family. It is a non-receptor kinase involved in actin reorganization, and in cell proliferation, survival, apoptosis, and cell adhesion in response to growth factors and interleukins. Another key functional role of Bmx is in TNF-alpha induced angiogenesis. Phosphorylation at Y566 by autocatalysis or by the protein-tyrosine kinase Src leads to activation and is required for STAT3 phosphorylation by Bmx. Src-induced Bmx phosphorylation could be almost completely inhibited with a Y566F mutation. Bmx has been demonstrated to prevent apoptosis of nasopharyngeal carcinomas.
