Product Name:
BRD2-pS37
Product Number:
ab-pk546
Target Full Name: Bromodomain-containing protein-serine kinase 2
Target Alias: KIAA9001; O27.1.1; Protein RING3; RING3; A2AAU0; B0V072; B0V073
Product Type Specific: BRD2 protein kinase phosphosite-specific antibody
Antibody Code: PK546
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S37
Protein UniProt: P25440
Protein SigNET: BRD2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human BRD2 sequence peptide Cat. No.: PE-04AIU99
Antibody Immunogen Sequence: RKP(pS)LLY(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R34 to Y40; Near the N-terminus of the protein. The effect of S37 phosphorylation is unclear. This is a major site of phosphorylation for BRD2 (≥341 reports by mass spectrometry in PhosphositePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-110 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactive proteins evident in sea star oocytes.
Related Product 1: BRD2 (34-40) pS37 antibody blocking peptide (Cat. No.: PE-04AIU99)
Scientific Background: BRD2 (Bromodomain-containing protein 2) is a key member of the BET (Bromodomain and Extra-Terminal motif) protein family that acts as an epigenetic reader. It is a protein-serine/threonine kinase of the Atypical group and BRD family, and plays a significant role in adipogenesis and obesity regulation. It is a transcriptional regulator that associates with transcription complexes and acetylated chromatin during mitosis. It plays a role in regulation of transcription through chromatin remodeling and nucleosome assembly. It binds to acetylated lysine residues on histones, regulating gene transcription, cell cycle progression, and chromatin organization. As a transcriptional activator, it often interacting with transcription factors like E2F1 to promote cell cycle progression. It contains two N-terminal bromodomains (BD1 and BD2) that bind to acetylated histone tails (specifically H4K12) and a C-terminal region (NET domain) that mediates protein-protein interactions. Mutations on multiple sites on BRD2 lead to loss of homodimerization. Overexpression of BRD2 is associated with lymphomas, and it is linked to juvenile myoclonic epilepsy. This description may include information annotated by UniProt and/or Google AI.