Product Name:
Bmx-Y40
Product Number:
ab-pk545
Target Full Name: Bone marrow X protein-tyrosine kinase
Target Alias: BMX NON-receptor tyrosine kinase; Bone marrow kinase BMX; Cytoplasmic BMX; Epithelial and endothelial tyrosine kinase; ETK; Kinase Etk; NTK38; Protein tyrosine kinase BMX; PSCTK3; ENSG00000102010
Product Type Specific: Bmx protein kinase phosphosite-specific antibody
Antibody Code: PK545
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y40
Protein UniProt: P51813
Protein SigNET: Bmx
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Bmx (Etk) sequence peptide Cat. No.: PE-04AIT95
Antibody Immunogen Sequence: NLS(pY)YEY(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues N37 to Y43; in the PH domain domain. Y40 phosphorylation stimulates phosphotransferase activity and regulates cell motility, cytoskeletal reorganization, intracellular location, and induces interaction with FAK. This phosphorylation site has not apparently been detected by mass spectrometry.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Mouse | Platypus.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 75-80 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Cross-reactivities are also seen with 4 other proteins of ~70 to 130 kDa in over night serum-starved Jurkat cells. Weakly cross reacts with phospho-ERK1 in sea star oocytes.
Related Product 1: Bmx (37-43) pY40 antibody blocking peptide (Cat. No.: PE-04AIT95)
Scientific Background: Bmx (Bone Marrow Kinase on X chromosome; Etk) is a protein-tyrosine kinase of the TK group and Tec family. It is a non-receptor kinase involved in actin reorganization, and in cell proliferation, survival, apoptosis, and cell adhesion in response to growth factors and interleukins. It regulates proinflammatory cytokine production. Another key functional role of Bmx is in TNF-alpha induced angiogenesis. It features an N-terminal pleckstrin homology (PH) domain for membrane localization, a SH3-SH2 domain for protein interaction, and a highly conserved C-terminal kinase domain. Phosphorylation at Tyr-566 by autocatalysis or by the protein-tyrosine kinase Src leads to activation and is required for STAT3 phosphorylation by Bmx. Src-induced Bmx phosphorylation could be almost completely inhibited with a Y566F mutation. Bmx is also a key player in PI3K/Akt pathways, and it directly phosphorylates and activates target proteins like BAK. It is predominantly found in hematopoietic cells, endothelial cells, and epithelial cells. Bmx plays a critical role in TNF-induced tumour vessel formation, and is up-regulated in various cancers (e.g., prostate, breast, gliomas), aiding in survival, tumour growth, and resistance to apoptosis (e.g., in nasopharyngeal carcinomas). This description may include information annotated by UniProt and/or Google AI.