Product Name:
Blk-pY389
Product Number:
ab-pk544
Target Full Name: B lymphoid tyrosine kinase
Target Alias: P55-Blk
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK543
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y389
Protein UniProt: P51451
Protein SigNET: P51451
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Blk sequence peptide Cat. No.: PE-04AIP90
Antibody Immunogen Sequence: DSE(pY)TAQ(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D386 to Q392; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues D386 to Q392; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 and Jurkat cells, except for possibly an insulin-stimulated ~60 kDa protein.
Related Product 1: Blk-pY389 blocking peptide
Related Product 2: Blk-pY187 phosphosite-specific antibody (Cat. No.: AB-PK541)
Related Product 3: Blk-pY188 phosphosite-specific antibody (Cat. No.: AB-PK542)
Scientific Background: Blk is a protein-tyrosine kinase that is a member of the TK group of protein kinases in the Src family. It has been reported that Blk is expressed specifically in the B cell lineage and plays a role in signal transduction pathway that is restricted to B lymphoid cells. However, this kinase has low to reasonably high expression (e.g. skeletal muscle, kidney, thyroid, breast) in most tested human tissues except in the brain and spinal cord where it is poorly expressed. Orthologues of Blk are highly conserved in mammals and birds. Blk is inhibited by phosphorylation at Y501. Blk has been linked with the development of colorectal adenocarcinomas. Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of Blk.