Product Name:
BMPR2-pS375
Product Number:
ab-pk544
Target Full Name: Bone morphogenetic protein receptor type-2
Target Alias: BMP type II receptor; BMPR3; BMPR-II; BMR2; Bone morphogenetic protein receptor type II precursor; Bone morphogenetic protein receptor, type II; BRK-3; PPH1; T-ALK
Product Type Specific: BMPR2 protein kinase phosphosite-specific antibody
Antibody Code: PK544
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S375
Protein UniProt: Q13873
Protein SigNET: BMPR2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human BMPR2 sequence peptide Cat. No.: PE-04AIS99
Antibody Immunogen Sequence: AAI(pS)EVG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues A372 to G378; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S375 phosphorylation is predicted to be stimulatory for phosphotransferase activity. This is one of the minor in vivo phosphorylation sites in BMPR2 (≥4 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Mouse | Zebra fish |.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.
Related Product 1: BMPR2 (372-378) pS375 antibody blocking peptide (Cat. No.: PE-04AIS99)
Scientific Background: BMPR2 (bone morphogenetic protein receptor type II) is a protein-serine/threonine kinase of the TKL group and STKR family. It is a transmembrane receptor kinase that is a member of the bone morphogenetic protein (BMP) receptor family. It features an extracellular domain for binding, a transmembrane domain, and an intracellular kinase domain. BMPR2 functions to bind BMPs, which are named for their role in endochondral bone formation and embryogenesis. It is activated by binding bone morphogenetic proteins such as BMP2, BMP4 and BMP7. This type II receptor phosphorylates and activates type I receptors which autophosphorylate, then phosphorylate SMAD (1, 5 and 8) transcriptional regulators. It is important ffor embryogenesis, particularly endochondral bone formation and skeletal patterning. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. Mutations in the BMPR2 gene are the leading cause of hereditary pulmonary arterial hypertension (PAH). Loss of function leads to vascular smooth muscle proliferation and excessive cellular proliferation. This description may include information annotated by UniProt and/or Google AI.