Product Name:
Blk-pY389
Product Number:
ab-pk543
Target Full Name: B lymphoid tyrosine kinase
Target Alias: EC 2.7.10.2; P55-BLK
Product Type Specific: BLK protein kinase phosphosite-specific antibody
Antibody Code: PK543
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y389
Protein UniProt: P51451
Protein SigNET: Blk
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Blk sequence peptide Cat. No.: PE-04AIP90
Antibody Immunogen Sequence: DSE(pY)TAQ(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D386 to Q392. Y389 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is one of the major sites of phosphorylation of BLK by mass spectrometry studies (≥72 reports).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells. Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in A431 and Jurkat cells, except for possibly an insulin-stimulated ~60 kDa protein.
Related Product 1: Blk (386-392) pY389 antibody blocking peptide (Cat. No.: PE-04AIP90)
Related Product 2: Blk Y187 phosphorylation antibody (Cat. No.: AB-PK541)
Related Product 3: Blk Y188 phosphorylation antibody (Cat. No.: AB-PK542)
Scientific Background: Blk is a protein-tyrosine kinase that is a member of the TK group of protein kinases in the Src family. Blk is expressed in cells of the B cell lineage and plays a role in B-lymphocyte activation, proliferation, and differentiation. It specifically phosphorylates CD79A, CD79B, and immunoglobulin G receptors (FCGR2A/B/C). Blk protein is predominantly expressed in B-lymphocytes, lymphoid organs (spleen, tonsil), and certain other tissues including pancreatic islets. However, this kinase has low to reasonably high expression (e.g. skeletal muscle, kidney, thyroid, breast) in most tested human tissues except in the brain and spinal cord where it is poorly expressed. Blk, along with other Src kinases like Lyn and Fyn, is activated upon B-cell receptor (BCR) signalling. It also contributes to B-cell receptor (pre-BCR)-mediated NF-kappa-B activation and mediates Btk activation. Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of Blk. Orthologues of Blk are highly conserved in mammals and birds. Blk is inhibited by phosphorylation at Tyr-501. It has been identified as a modulator of beta-cell function; its deficit is linked to MODY11 (Maturity-Onset Diabetes Of The Young, Type 11). Genetic variants in the Blk gene are associated with autoimmune diseases such as systemic lupus erythematosus (SLE) and systemic sclerosis (SSc). Blk has been linked with the development of colorectal adenocarcinomas and cutaneous T-cell lymphoma (CTCL). This description may include information annotated by UniProt and/or Google AI.