Product Name:
Blk-pY187
Product Number:
ab-pk542
Target Full Name: B lymphoid tyrosine kinase
Target Alias: P55-Blk
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK541
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y187
Protein UniProt: P51451
Protein SigNET: P51451
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Blk sequence peptide Cat. No.: PE-04AIQ99
Antibody Immunogen Sequence: EGG(pY)YIS(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E184 to S190; In the SH2 domain. One of the two major in vivo phosphorylation sites in Blk.
Production Method: Corresponds to amino acid residues E184 to S190; In the SH2 domain. One of the two major in vivo phosphorylation sites in Blk.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.15/1.0 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-weak immunoreactivity of a target-sized protein by Western blotting in Jurkat cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 55-60 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in phenylarsine oxide (PAO)+vanadate-treated HeLa cells, EGF-treated A431 cells and insulin-treated MCF7 cells, when these cells were homogenized in SDS-PAGE sample buffer.
Related Product 1: Blk-pY187 blocking peptide
Related Product 2: Blk-pY188 phosphosite-specific antibody (Cat. No.: AB-PK542)
Related Product 3: Blk-pY389 phosphosite-specific antibody (Cat. No.: AB-PK543)
Scientific Background: Blk is a protein-tyrosine kinase that is a member of the TK group of protein kinases in the Src family. It has been reported that Blk is expressed specifically in the B cell lineage and plays a role in signal transduction pathway that is restricted to B lymphoid cells. However, this kinase has low to reasonably high expression (e.g. skeletal muscle, kidney, thyroid, breast) in most tested human tissues except in the brain and spinal cord where it is poorly expressed. Orthologues of Blk are highly conserved in mammals and birds. Blk is inhibited by phosphorylation at Y501. Blk has been linked with the development of colorectal adenocarcinomas. Stimulation of resting B-lymphocytes with antibodies to surface immunoglobulin (sIgD or sIgM) induces activation of Blk.
