Product Name:
BARK1-Y356
Product Number:
ab-pk538
Target Full Name: Beta-adrenergic receptor kinase 1
Target Alias: ADRBK1; Adrenergic, beta, receptor kinase 1; ARBK1; BARK; BARK1; Beta-adrenergic receptor kinase 1; Beta-ARK-1; EC 2.7.11.15; FLJ16718; G- protein coupled receptor kinase 2; Kinase GRK2
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK537
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y356
Protein UniProt: P25098
Protein SigNET: P25098
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human BARK1 (GRK2, ADRBK1) sequence peptide Cat. No.: PE-04ACJ85
Antibody Immunogen Sequence: THG(pY)MAP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T353 to P359; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues T353 to P359; In the protein kinase catalytic domain activation T loop region between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in FBS-treated Jurkat cells and weakly in HeLa cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 78-85 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No medium to strong cross-reactivities with other proteins in Jurkat cells, HeLa cells, and sea star oocytes.
Related Product 1: BARK1-Y356 blocking peptide
Related Product 2: BARK1-pS670 phosphosite-specific antibody (Cat. No.: AB-PK536)
Scientific Background: BARK1 (GRK2, ADRBK1) is a protein-serine/threonine kinase of the AGC group and GRK family. It is activated by G-beta/gamma subunit complex. Phosphorylation of S29 increases phosphotransferase activity. Phosphorylation of S670 inhibits G-beta/gamma-mediated activation of GRK2. Phosphorylation of Y13, Y86, Y92 and S685 increases interaction with G-alpha(q) and may induce protein degradation.
