Product Name:
AurKB-pT232
Product Number:
ab-pk532
Target Full Name: Aurora Kinase B (serine/threonine protein kinase 12)
Target Alias: AIK2; AIM1; AIM-1; ARK2; AurkB; Aurora,IPL1-related kinase 2; Aurora-2; Aurora-B; STK12; AurkB; IPL1; STK12; STK5; CCDS11134.1; ENSG00000178999
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK531
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T232
Protein UniProt: Q96GD4
Protein SigNET: Q96GD4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human AurKB (Aurora B, AIM-1) sequence peptide Cat. No.: PE-04AIM90
Antibody Immunogen Sequence: RRK(pT)MCG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R229 to G235; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Production Method: Corresponds to amino acid residues R229 to G235; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-45 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Medium immunoreactivity on protein dot blots with recombinant human AurKA and weak with AurKC.
Related Product 1: AurKB-pT232 blocking peptide
Related Product 2: AurKB pan-specific antibody (Cat. No.: AB-NK193-2P)
Related Product 3: AurKB-1 pan-specific antibody (Cat. No.: AB-NK193-2)
Related Product 4: AurKB-2 pan-specific antibody (Cat. No.: AB-NK193-3)
Related Product 5: AurKB-3 pan-specific antibody (Cat. No.: AB-NK193-4)
Related Product 6: AurKB-pS227 phosphosite-specific antibody (Cat. No.: AB-PK530)
Scientific Background: AurB (AurKB, AIK2) is a protein-serine/threonine kinase of the Other group and AUR family. It serves as a component of the chromosomal passenger complex (CPC) that plays a critical role in regulating mitosis. AurB may be a tumour requiring protein (TRP). Its low rate of mutation and down-regulation in human cancers supports it identification as a tumour-requiring protein, and as a target for cancer drug development. However, AurB is overexpressed in some colorectal cancers and other tumour cell lines, which was thought to lead to multinuclearity, increased ploidy, and a predisposition to cancer. Through its dominant-negative effect on cytokinesis, disruptive regulation of its expression is potentially responsible for the perturbing the chromosomal integrity in cancer cells. A K106R mutation leads to loss of phosphotransferase activity, and severely impairs mitosis.
