Product Name:
AurKB-pS227
Product Number:
ab-pk530
Target Full Name: Aurora Kinase B (serine/threonine protein kinase 12)
Target Alias: AIK2; AIM1; AIM-1; ARK2; AurkB; Aurora,IPL1-related kinase 2; Aurora-2; Aurora-B; STK12; AurkB; IPL1; STK12; STK5; CCDS11134.1; ENSG00000178999
Product Type Specific: AurKB protein kinase phosphosite-specific antibody
Antibody Code: PK530
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S227
Protein UniProt: Q96GD4
Protein SigNET: AurKB
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human AurKB (Aurora B, AIM-1) sequence peptide Cat. No.: PE-04AAR95
Antibody Immunogen Sequence: VAHAP(pS)L(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues H224 to L228; in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S227 phosphorylation is predicted to be stimulatory for phosphotransferase activity. Phosphorylation induces interaction with INCENP. This is a minor in vivo phosphorylation site in AurKB (≥11 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). AurKB is known to be phosphorylated at this site in vitro by AurKB and PKCe.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in sea star oocyte. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-45 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human AurKC, but no immunoreactivity with AurKA. No cross-reactive proteins detected in A549 and HeLa cells. A strong 36 kDa cross-reactive protein was detected in sea star oocytes, and a strong 28 kDa cross-reactive protein in Jurkat cells.
Related Product 1: AurB (222-228) pS227 antibody blocking peptide (Cat. No.: PE-04AAR95)
Related Product 2: AurKB expression antibody (Cat. No.: AB-NK193-2P)
Related Product 3: AurKB expression antibody (Cat. No.: AB-NK193-2)
Related Product 4: AurKB expression antibody (Cat. No.: AB-NK193-3)
Related Product 5: AurKB expression antibody (Cat. No.: AB-NK193-4)
Related Product 6: AurKB T232 phosphorylation antibody (Cat. No.: AB-PK531)
Related Product 7: AurKA expression antibody (Cat. No.: AB-NK008-3)
Related Product 8: AurKA T287+T288 phosphorylation antibody (Cat. No.: AB-PK529)
Related Product 9: AurKC expression antibody (Cat. No.: AB-NK009-2)
Related Product 10: AurKC expression antibody (Cat. No.: AB-NK009-3)
Scientific Background: AurB (AurKB, AIK2) is a 39 kDa protein-serine/threonine kinase of the Other group and AUR family. It serves as a component of the chromosomal passenger complex (CPC) that plays a critical role in regulating mitosis. It localizes dynamically to different cellular structures during division: chromosomes in prophase, the centromere in prometaphase/metaphase, and the central mitotic spindle in anaphase. It is crucial for proper chromosome alignment, error-free segregation, and the onset of cytokinesis. AurB phosphorylates Histone H3 at Ser-10, which facilitates chromosome condensation. It also phosphorylates other CPC components (INCENP, Survivin, Borealin) and substrates involved in cell division, including CENP-A and vimentin. AurB may be a tumour requiring protein (TRP). Its low rate of mutation and down-regulation in human cancers supports it identification as a tumour-requiring protein, and as a target for cancer drug development. However, AurB is overexpressed in some colorectal cancers and other tumour cell lines, which was thought to lead to multinuclearity, increased ploidy, and a predisposition to cancer. Through its dominant-negative effect on cytokinesis, disruptive regulation of its expression is potentially responsible for the perturbing the chromosomal integrity in cancer cells. A K106R mutation leads to loss of phosphotransferase activity, and severely impairs mitosis. This description may include information annotated by UniProt and/or Google AI.