Product Name:
ANKRD3-pS438
Product Number:
ab-pk523
Target Full Name: Ankyrin repeat domain protein-serine kinase 3
Target Alias: ANKK2; Ankyrin repeat domain protein 3; DIK; Kinase ANKRD3; PKC-delta-interacting protein kinase; PKC-regulated kinase PKK; Receptor-interacting serine-threonine kinase 4; RIP4; RIPK4; DIK
Product Type Specific: ANKRD3 protein kinase phosphosite-specific antibody
Antibody Code: PK523
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S438
Protein UniProt: P57078
Protein SigNET: ANKRD3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ANKRD3 sequence peptide Cat. No.: PE-04AIH99
Antibody Immunogen Sequence: LSL(pS)FER(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L435 to R441; in the region between the kinase catalytic and Ank_2 domains. The effect of S438 phosphorylation is unclear. It is the major site of in vivo phosphorylation in ANKRD3 with ≥12 reports by mass spectrometry in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-100 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactive proteins detected in A431 cells, HeLa cells, and sea star oocytes.
Related Product 1: ANKRD3 (435-441) pS438 antibody blocking peptide (Cat. No.: PE-04AIH99)
Scientific Background: ANKRD3 (RIPK4) is a 86-112 kDa protein-serine/threonine kinase of the TKL group and RIPK family. It is involved in keratinocyte differentiation, skin development, and wound repair. It is highly expressed in skin and hair follicles. It features an N-terminal kinase domain, a central region with 10 ankyrin-like repeats, and a C-terminal domain, and is often found at membranes. ANKRD3 interacts with Protein Kinase C (PKC). Phosphorylation at Thr-184 increases the phosphotransferase activity. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. Mutations or dysfunction are associated with skin disorders, and it has context-dependent roles as both a potential oncogene or tumour suppressor. This description may include information annotated by UniProt and/or Google AI.