Product Name:
AMPKa1-pT183+pS184
Product Number:
ab-pk521
Target Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
Target Alias: 5'-AMP-activated protein kinase, catalytic alpha-1 chain; AAPK1; Acetyl-CoA carboxylase kinase; AMPK alpha-1 chain; AMPK, alpha, 1; AMPK-alpha1; HMG-CoA reductase kinase; HMG-CoA reductase kinase; PRKAA1; Protein kinase, AMP-activated, alpha 1 catalytic subunit; MGC33776; MGC57364; CCDS3932.1; ENSG00000132356
Product Type Specific: AMPKa1 protein kinase phosphosite-specific antibody
Antibody Code: PK521
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T183+S184
Protein UniProt: Q13131
Protein SigNET: AMPKa1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human AMPKa1 (PRKAA1) sequence peptide Cat. No.: PE-04AIG99
Antibody Immunogen Sequence: FLR(pS)(pT)CG(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues F180 to G186; In protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T183 and S184 stimulate phosphotransferase activity. This is one of the major in vivo phosphorylation sites in AMPKa1 (T183 has ≥ 48 reports mass spectrometry and S184 has ≥20 reports, respectively, in PhosphoSitePlus. AMPKa1 is known to be phosphorylated in vitro at T183 by AMPKa1, BRSK1, BRSK2, CaMK2b, CAMKK1, CaMKK2, LKB1, LKB1, MLK3, MST1, MST2, MST3, and MST4.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in insulin-stimulated MCF7 cells. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Rat | Mouse | Frog | Zebra fish | Nematode worm | Budding yeast.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-65 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: No significant cross-reactivities detected in HeLa except phenylarsine oxide (PAO) increase detection of a 27 kDa protein; in Jurkat cells, phenylarsine oxide (PAO) decrease 100 kDa protein and phenylarsine oxide (PAO) increase 15 kDa protein. This antibody appears to cross-reacts with CDK1 in sea star oocytes.
Related Product 1: AMPKa1 (180-186) pT183+pS184 antibody blocking peptide (Cat. No.: PE-04AIG99)
Related Product 2: AMPKSelectide kinase substrate peptide (Cat. No.: PE-01BGH95)
Related Product 3: AMPKa1 expression antibody (Cat. No.: AB-NK259-1)
Related Product 4: AMPKa1 expression antibody (Cat. No.: AB-NK259-2)
Related Product 5: AMPK T172 phosphorylation antibody (Cat. No.: AB-PK897)
Related Product 6: AMPKa1 T490 phosphorylation antibody (Cat. No.: AB-PK918)
Related Product 7: AMPKa2 expression antibody (Cat. No.: AB-NK260-1)
Related Product 8: AMPKa2 expression antibody (Cat. No.: AB-NK260-2)
Related Product 9: AMPKa2 S377 phosphorylation antibody (Cat. No.: AB-PK522)
Related Product 10: AMPKa2 Y436 phosphorylation antibody (Cat. No.: AB-PK919)
Scientific Background: AMPKa1 (PRKAA1) is a 64 kDa protein-serine/threonine kinase of the CAMK group and CAMKL family. AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-183 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signalling pathways in response to adiponectin, leptin and CAMKK-beta. Phosphorylation at Thr-174 by CAMKK1 and CAMKK2 increases phosphotransferase activity, triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. AMPKa1 regulates cell growth by inhibiting mTORC1 and promoting autophagy via phosphorylation of TSC2, RPTOR, and ULK1. It inhibits fatty acid/cholesterol synthesis (by direct phosphorylation of metabolic enzymes, such as ACACA and HMGCR) and promotes glucose uptake (via GLUT4 translocation) and fatty acid oxidation. It is activated by therapeutic compounds like metformin or resveratrol. Motor neurons of ALS patients exhibiting cytoplasmic TDP-43 mislocalization showed an increased level of AMPK activation. It is also associated with metabolic disorders, fatty liver disease, and tumor regulation. This description may include information annotated by UniProt and/or Google AI.