Product Name:
Akt1-pY315
Product Number:
ab-pk516
Target Full Name: RAC-alpha serine/threonine-protein kinase
Target Alias: Akt; Akt1; Akt1 kinase; C-Akt; Kinase Akt1; PKB; PKB-alpha; PRKBA; V-akt murine thymoma viral oncogene 1; Protein kinase B; RAC; RAC-alpha serine,threonine kinase; RAC-alpha serine/threonine kinase; RAC-PK-alpha; PKBA; hCG_96740; Akt1_NEW; Akt; MGC99656; CCDS9994; B0LPE5; B2RAM5; B3KVH4; B3KXD7; B7Z5R1; Q9BV07; ENSG00000142208
Product Type Specific: Akt1 protein kinase phosphosite-specific antibody
Antibody Code: PK516
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y315
Protein UniProt: P31749
Protein SigNET: Akt1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Akt1 (PKBa) sequence peptide Cat. No.: PE-04ACH99
Antibody Immunogen Sequence: TPE(pY)LAP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues T312 to P318. Y315 phosphorylation is predicted to be inhibitory for phosphotransferase activity, although it has been suggested to be activatory. This phosphosite is located in the kinase activation loop just before the catalytic subdomain VIII. There has been ≥15 reports of its phosphorylation in high throughput mass spectrometry in PhosphoSitePlus. Akt1 is known to be phosphorylated at this site in vitro by Brk, Ret, and Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots. Very strong immunoreactivity with recombinant human Akt1 on protein dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Dog | Rat | Mouse | Platypus | Frog | Fruit fly | Nematode worm.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 57-62 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: Strong immunoreactivity on protein dot blots with Akt2 and Akt3, which feature identical phosphosite sequences. No cross-reactive proteins detected in HepG2 cells, MCF7 cells or seastar oocytes.
Related Product 1: Akt1 (312-318) pY315 antibody blocking peptide (Cat. No.: PE-04ACH99)
Related Product 2: Akt1 expression antibody (Cat. No.: AB-NK129-3)
Related Product 3: Akt1 expression antibody (Cat. No.: AB-NK129-4)
Related Product 4: Akt1 expression antibody (Cat. No.: AB-NK129-5)
Related Product 5: Akt1 T308 phosphorylation antibody (Cat. No.: AB-PK515)
Related Product 6: Akt1 Y326 phosphorylation antibody (Cat. No.: AB-PK517)
Related Product 7: Akt1 T443 phosphorylation antibody (Cat. No.: AB-PK973)
Related Product 8: Akt1 S473 phosphorylation antibody (Cat. No.: AB-PK869)
Related Product 9: Akt1 S473 phosphorylation antibody (Cat. No.: AB-PK958)
Related Product 10: Akt2 expression antibody (Cat. No.: AB-NK130-4)
Scientific Background: Akt1 (PKBA) is a protein-serine/threonine kinase of the AGC group and Akt family. It is normally inactive in the cytoplasm. When PI3K is activated by extracellular growth factors, Akt1 is recruited to the cell membrane and phosphorylated by PDK1 (T308) , mTOR (at S473) and other protein kinases to become active. Phosphorylation of T72, S124, S129, S246, T308, Y315,Y326, T450, S473 and Y474 all contribute to increased Akt1 phosphotransferase activity. TRAF6-induced 'K63'-linked Akt1 ubiquitination is critical for phosphorylation and activation. Phosphorylation of T34 inhibits phosphotransferase activity. Akt1 activation leads to the induction of survival, proliferation, angiogenesis and metabolism pathways. Akt1 is also involved in cell-cycle progression via its inhibitory phosphorylation of cyclin-dependent kinases and feedback activatory phosphorylation of mTOR. It phosphorylates over 100 known substrates to inhibit apoptosis and stimulate anabolic processes. PAK1 consists of an N-terminal pleckstrin homology (PH) domain, a central kinase domain, and a C-terminal regulatory domain. This kinase is highly expressed in most tested human tissues. Orthologues of Akt are conserved in a wide range of animals, plants and unicellular eukaryotes. Overactive or overexpressed Akt1 is implicated in various cancers (e.g., lymphomas, breast, lung, colorectal, pancreatic and prostate) and is linked to psychiatric conditions like schizophrenia when associated with cannabis use. This description may include information annotated by UniProt and/or Google AI.