Product Name:
AAK1-pS637
Product Number:
ab-pk503
Target Full Name: AP2-associated protein kinase 1
Target Alias: Adaptor-associated kinase 1; AP2 associated kinase 1; DKFZp686K16132; KIAA1048; Kinase AAK1
Product Type Specific: AAK1 protein kinase phosphosite-specific antibody
Antibody Code: PK503
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S637
Protein UniProt: Q2M2I8
Protein SigNET: AAK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human AAK1 sequence peptide Cat. No.: PE-04ALP99
Antibody Immunogen Sequence: RIL(pS)DVT(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues R634 to T640; in the C-terminal half of the kinase after the catalytic domain. The major site of phosphorylation of AAK1. The effect of S637 phosphorylation is unclear. This is major site of phosphorylation of AAK1 by mass spectrometry studies (>262 reports recorded in PhosphoSitePlus). Regulates dendrite and spine development in neurons.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity of a target-sized protein by Western blotting in serum-starved Jurkat cells treated with for 10 minutes with 10% fetal calf serum. Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 90-95 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: Prominent immunreactivities in FBS-treated Jurkat cells at 75 to 120 kDa range.
Related Product 1: AAK1 (634-640) pS637 antibody blocking peptide (Cat. No.: PE-04ALP99)
Scientific Background: AAK1 is a protein-serine/threonine kinase of the Other group and NAK family. It stimulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) to ensure high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. AAK1 also targets the phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. It phosphorylates NUMB and promotes NUMB localization to endosomes. It also binds and stabilizes the activated form of NOTCH1 to increases its localization in endosomes. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. This description may include information annotated by UniProt and/or Google AI.