Product Name:
A6r-pY309
Product Number:
ab-pk502
Target Full Name: Twinfilin, actin-binding protein, 2; Protein tyrosine kinase 9-like
Target Alias: TWF2; PTK9L
Product Type Specific: A6r protein kinase phosphosite-specific antibody
Antibody Code: PK502
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y309
Protein UniProt: Q6IBS0
Protein SigNET: A6r
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human A6r (Twinfin-2) sequence peptide Cat. No.: PE-04ACE99
Antibody Immunogen Sequence: EFL(pY)DEV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E306 to V312; in the end of the Cofilin_ADF domain. The major site of phosphorylation of A6r. The effect of Y309 phosphorylation is unclear. It is the major site of phosphorylation of A6r by mass spectrometry studies (>524 reports).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Chicken | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 39-40 kDa.
Antibody Cross Reactivity: No significant cross-reactive proteins detected in seastar oocytes.
Related Product 1: A6r (306-312) pY309 antibody blocking peptide (Cat. No.: PE-04ACE99)
Related Product 2: A6-pY309 phosphosite-specific antibody (Cat. No.: AB-PK501)
Scientific Background: The A6 family of atypical kinases, which includes human A6 and A6r genes and their homologs, are often classified as tentative pseudogenes or putative protein kinases, with evidence for actual kinase activity being limited. Human A6 (Twinfilin-1) was initially reported to have tyrosine phosphotransferase activity. However, the closely related A6r (Twinfilin-2) was shown to bind ATP but failed to demonstrate kinase activity, indicating that some members may be functionally distinct or inactive (pseudogenes). These proteins have evolutionary homologs in flies, worms, and yeast. A6r was identified through its interaction with protein kinase C zeta (PKCz) in two-hybrid studies. A6r appears to be an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, probably by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. It seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. This description may include information annotated by UniProt and/or Google AI.