Product Name:
A6-pY309
Product Number:
ab-pk502
Target Full Name: Twinfilin, actin-binding protein, 1; Protein tyrosine kinase 9;
Target Alias: TWF1; PTK9
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK501
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y309
Protein UniProt: Q12792
Protein SigNET: Q12792
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human A6 (Twinfilin-1) sequence peptide Cat. No.: PE-04AGX99
Antibody Immunogen Sequence: DFL(pY)EEV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D306 to V312; In the end of the Cofilin_ADF domain. The major site of phosphorylation of A6.
Production Method: Corresponds to amino acid residues D306 to V312; In the end of the Cofilin_ADF domain. The major site of phosphorylation of A6.
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 39-40 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in seastar oocytes.
Related Product 1: A6-pY309 blocking peptide
Related Product 2: A6r-pY309 phosphosite-specific antibody (Cat. No.: AB-PK502)
Scientific Background: A6 appears to be an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, probably by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. It seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.
