Product Name:
Raf-A-pY302
Product Number:
ab-pk501
Target Full Name: A-Raf proto-oncogene serine/threonine-protein kinase
Target Alias: ARAF; A-RAF; ARAF1; A-raf-1; Kinase A-Raf; PKS; PKS2; Proto-oncogene Pks; V-raf murine sarcoma 3611 viral oncogene; RP1-230G1.1-005; RP1-230G1_1; A6NIT1; B4DMG5; Q5H9B2; Q96II5ENSG00000078061
Product Type Specific: Protein kinase phosphosite-specific antibody
Antibody Code: PK500
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y302
Protein UniProt: P10398
Protein SigNET: P10398
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Raf-A (ARaf) sequence peptide Cat. No.: PE-04AFW99
Antibody Immunogen Sequence: SGY(pY)WEV(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues S299 to V305; Just before kinase catalytic domain
Production Method: Corresponds to amino acid residues S299 to V305; Just before kinase catalytic domain
Antibody Modification: Protein kinase phosphosite-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 63-70 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactive proteins detected in HeLa, Jurkat and seastar oocytes.
Related Product 1: A-Raf-pY302 blocking peptide
Related Product 2: Raf-A-1 pan-specific antibody (Cat. No.: AB-NK205-1)
Related Product 3: Raf-A-2 pan-specific antibody (Cat. No.: AB-NK205-3P)
Related Product 4: Raf-A-3 pan-specific antibody (Cat. No.: AB-NK205-4P)
Related Product 5: Raf-A-CT (RafA-4) pan-specific antibody (Cat. No.: AB-NK205)
Scientific Background: A-Raf (RafA) is a protein-serine/threonine kinase of the TKL group and RAF family. It is involved in the transduction of mitogenic signals from the cell membrane receptors via Ras and MEK1/2 to the nucleus. It has been implicated in playing a role in the postsynaptic responses of hippocampal neuron. Phosphorylation of S214, S257, S262, S264, Y301, Y302, S432, T442, T452, T455 and S582 increases phosphotransferase activity. It is inhibited by Nexavar/Sorafenib (IC50>50 nM). A-Raf may be an oncoprotein (OP) based on its similarity to Raf1 and B-Raf. However, it does not display a higher than normal rate of mutation than most proteins in human tumours. The active form of the protein kinase acts to promote tumour cell proliferation.
