Product Name:
PKR1-pT446
Product Number:
ab-pk133
Target Full Name: Protein-serine/threonine kinase Interferon-induced, double-stranded RNA-activated protein kinase Thr-446 phosphosite
Target Alias: ADRB2; E2AK2; EC 2.7.11.1; EIF2aK; EIF2AK1; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-inducible RNA-dependent protein kinase; Kinase PKR; P1,eIF-2A protein kinase; P1/eIF-2A protein kinase; P68 kinase; PRKR; TIK
Product Type Specific: Phosphosite-specific polyclonal antibody for human PKR T446 phosphosite
Antibody Code: PK132
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T446
Protein UniProt: P19525
Protein SigNET: P19525
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Peptide with amino acid sequence surrounding the human PKR Thr-446 phosphosite (Lys-Arg-pThr-Arg-Ser)
Antibody Immunogen Sequence: KRpTRS
Antibody Immunogen Description: 5 amino acid phosphopeptide corresponding to the human PKR1 T446 phosphosite
Production Method: 5 amino acid phosphopeptide corresponding to the human PKR1 T446 phosphosite
Antibody Modification: Phosphosite-specific polyclonal antibody for human PKR T446 phosphosite
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol
Storage Conditions: -20°C
Storage Stability: > 1 year
Product Use: Western blotting, Immunohistochemistry (IHC)
Antibody Dilution Recommended: 2 µg/mL
Antibody Potency: Very strong
Antibody Species Reactivity: Human, Mouse
Antibody Positive Control: T446 phosphorylated PKR1
Antibody Specificity: Medium
Antibody Cross Reactivity: Cross-reactivities observed with ~16, ~42, ~60 and ~120 kDa proteins.
Related Product 1: PKR1-pT446 phospho-specific antibody (Cat. No.: AB-PK132)
Related Product 2: PKR1-pT446 phospho-specific antibody (Cat. No.: AB-PK777)
Scientific Background: PKR a protein kinase of the PEK family. Upon binding double-stranded RNA (which can arise from certain viral infections) or heparin, it autophosphorylates at Thr-451 and become activated. It is also activated by interaction with HIV-1 Tat. PKR phosphorylates and inhibits the alpha subunit of eIF2 alpha (EIF2S1), which leads to an inhibition of the initiation of protein synthesis. It controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. PKR mediates apoptosis induced by many different stimuli, such as lipopolysaccharide (LPS), TNF-alpha, viral infection and serum starvation.
