Product Name:
JAK1-pY1022
Product Number:
ab-pk132
Target Full Name: Protein-tyrosine kinase JAK1 Tyr-1022 phosphosite
Target Alias: EC 2.7.10.2; JAK1A; Janus kinase 1
Product Type Specific: Phosphosite-specific polyclonal antibody for human JAK1 Y1022 phosphosite
Antibody Code: PK126
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1022
Protein UniProt: P23458
Protein SigNET: P23458
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Peptide with amino acid sequence surrounding the human JAK1 Tyr-1022 phosphosite (Lys-Glu-pTyr-Tyr-Thr)
Antibody Immunogen Sequence: KEpYYT
Antibody Immunogen Description: 5 amino acid phosphopeptide corresponding to the human JAK1 Y1022 phosphosite
Production Method: 5 amino acid phosphopeptide corresponding to the human JAK1 Y1022 phosphosite
Antibody Modification: Phosphosite-specific polyclonal antibody for human JAK1 Y1022 phosphosite
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol
Storage Conditions: -20°C
Storage Stability: > 1 year
Product Use: Western blotting, Immunohistochemistry (IHC)
Antibody Dilution Recommended: 2 µg/mL
Antibody Potency: Strong
Antibody Species Reactivity: Human, Mouse, Rat
Antibody Positive Control: Y1022 phosphorylated JAK1
Antibody Specificity: High
Antibody Cross Reactivity: Ocassionally, strong cross-reactivity with a ~23 kDa protein and weaker cross-reactivity with a ~50 kDa protein can be detected.
Related Product 1: JAK1-1 pan-specific antibody (Cat. No.: AB-NK084-3)
Related Product 2: JAK1-3 pan-specific antibody (Cat. No.: AB-NK084-5)
Scientific Background: JAK1 is a widely expressed non-receptor tyrosine-kinase involved in the interferon-alpha/beta and -gamma signal transduction pathways, and can bind directly to the Interleukin 2 receptor. It couples cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. It is known to interact with IL31RA, IFNAR2, JAKMIP1 (via FERM domain) and SHB. It is expressed at higher levels in primary colon tumors than in normal colon tissue, but its expression level in metastatic colon tumors is comparable to the level in normal colon tissue. It is also activated in B cell lines from patients with post-transplant lymphoproliferative disorder, and in a mouse Lck-driven T cell lymphoma. JAK family kinases feature two protein kinase domains. Piceatannol is a known JAK1 inhibitor.
