Product Name:
PKR1-pT446
Product Number:
ab-pk132
Target Full Name: Double-stranded RNA-dependent protein-serine kinase; Interferon-induced, double-stranded RNA-activated protein kinase
Target Alias: ADRB2; E2AK2; EIF2aK; EIF2AK1; EIF2AK2; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-inducible RNA-dependent protein kinase; P1,eIF-2A protein kinase; TIK; MGC126524; ENSG00000055332
Product Type Specific: PKR1 protein kinase phosphosite-specific antibody
Antibody Code: PK132
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T446
Protein UniProt: P19525
Protein SigNET: PKR1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Peptide with amino acid sequence surrounding the human PKR Thr-446 phosphosite
Antibody Immunogen Sequence: KR(pT)RS
Antibody Immunogen Description: 5 amino acid phosphopeptide corresponding to the human PKR1 T446 phosphosite.
Production Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol
Storage Conditions: -20°C
Storage Stability: > 1 year
Product Use: Western blotting, Immunohistochemistry (IHC)
Antibody Dilution Recommended: 2 µg/mL
Antibody Potency: Very strong
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee.
Antibody Positive Control: T446 phosphorylated PKR1
Antibody Specificity: Medium
Antibody Cross Reactivity: Cross-reactivities observed with ~16, ~42, ~60 and ~120 kDa proteins.
Related Product 1: PKR1-pT446 phospho-specific antibody (Cat. No.: AB-PK777)
Related Product 2: PKR T451 phosphorylation antibody (Cat. No.: AB-PK910)
Scientific Background: PKR (PRKR, EIF2AK1) is a protein-serine/threonine kinase of the Other group and PEK family. It has an antiviral activity on various ranges of DNA and RNA viruses such as hepatitis C virus, hepatitis B virus, measles virus, and herpes simplex virus 1. It is activated by autophosphorylation after binding to dsRNA (generated during virus infection) in the presence of ATP. Besides double stranded RNA (dsRNA), heparin is a potent activator of the kinase. Binding to dsRNA is required for dimerization leading to autophosphorylation in the activation loop and stimulation of function. Activity is markedly stimulated by manganese ions. Autophosphorylation at S83, T88, T89, T90, Y101, Y162, S242, T255, T258, Y293 T446, T451, and S456 increase the phosphotransferase activity of EIF2AK2. Once activated, PKR can phosphorylate translation initiation factor EIF2S1, which in turn will inhibit protein synthesis. It can be inhibited by vaccinia virus protein E3, possibly via dsRNA sequestering. It has a role in p53-mediated responses to genotoxic stress. Since the protein has a crucial role for inflammation activation, it can be a potential therapeutic target to treat inflammation. This description may include information annotated by UniProt and/or Google AI.