Product Name:
ErbB2-pY877
Product Number:
ab-pk125
Target Full Name: Receptor protein-tyrosine kinase ErbB2 Tyr-877 phosphosite
Target Alias: CD340; C-erbB-2; Epidermal growth factor receptor-related protein; ErbB2; HER2; HER-2; Kinase ErbB2; MLN 19; V-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); TKR1; hCG_28177; ENSG00000141736; Q9NP09; Q9UK79
Product Type Specific: ErbB2 protein kinase phosphosite-specific antibody
Antibody Code: PK125
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y877
Protein UniProt: P04626
Protein SigNET: ErbB2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Peptide with amino acid sequence surrounding human ErbB2 Tyr-877 phosphosite
Antibody Immunogen Sequence: TE(pY)HA
Antibody Immunogen Description: 5 amino acid phosphopeptide corresponding to the human ErbB2 Y877 phosphosite.
Production Method: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol
Storage Conditions: -20°C
Storage Stability: > 1 year
Product Use: Western blotting, Immunohistochemistry (IHC)
Antibody Dilution Recommended: 2 µg/mL
Antibody Potency: Very strong
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Zebra fish.
Antibody Positive Control: Y877 phosphorylated ErbB2
Antibody Specificity: Very high
Antibody Cross Reactivity: No strong cross-reactivities with other proteins.
Related Product 1: ErbBSubtide kinase substrate peptide (Cat. No.: PE-01BHG95)
Related Product 2: ErbB2 expression antibody (Cat. No.: AB-NK054-4P)
Related Product 3: ErbB2 expression antibody (Cat. No.: AB-NK054-4)
Related Product 4: ErbB2 expression antibody (Cat. No.: AB-NK054-5)
Related Product 5: ErbB2 expression antibody (Cat. No.: AB-NK054-6)
Related Product 6: ErbB2 expression antibody (Cat. No.: AB-NK054-7)
Related Product 7: ErbB2 Y735 phosphorylation antibody (Cat. No.: AB-PK614)
Related Product 8: ErbB2 Y877 phosphorylation antibody (Cat. No.: AB-PK615)
Related Product 9: ErbB2 Y1248 phosphorylation antibody (Cat. No.: AB-PK613)
Scientific Background: ErbB2 (HER2; Neu; CD340) is a 138-185 kDa protein-tyrosine kinase of the TK group and EGFR family. It features an extracellular ligand-binding domain (which binds no ligand), a transmembrane domain, and an intracellular tyrosine kinase domain. It is a kinase that function as a component of several membrane protein receptor complexes on the cell surface. Specifically, the ErbB2 protein functions in the regulation of the growth and stabilization of peripheral microtubules, as well as in the transcriptional activation of several rRNA genes (mediated by RNA polymerase I) that result in enhanced protein synthesis and subsequent cell growth. It is commonly expressed in most tested human tissues except in the brain and spinal cord, where it is more weakly expressed. Protein interaction is induced with phosphorylation of Tyr-1139 (with Grb2, Grb7 & Src), Tyr-1196 (with Crk), and Tyr-1222 (with CGI-27, Grb2 & Shc1). Phosphorylation of Tyr-1112 induces interaction with Cbl, which promotes ErbB2 degradation. Overexpression of unaltered ErbB2 coding sequences in NIH-3T3 cells results in cellular transformation and tumorigenesis. ErbB2 is amplified in 15-30% of primary human breast malignancies and overexpression of ErbB2 is associated with the most aggressive tumours that show uncontrolled proliferation, resistance to apoptosis and increased motility. ErbB2 has also been linked with the development of ovarian cancer, gastric and lung adenocarcinomas, and glioblastomas. Insertional mutagenesis studies in mice also support a role for this protein kinase in mouse cancer oncogenesis. This description may include information annotated by UniProt and/or Google AI.